Acosta-Urquidi J, Chase R
Can J Physiol Pharmacol. 1975 Oct;53(5):793-8. doi: 10.1139/y75-109.
The effects of delta9-tetrahydrocannabinol (delta9-THC) on action potentials were examined during intrasomatic recordings from the isolated buccal and parieto-visceral ganglia of Aplysia californica. When added to the saline solution bathing the preparation, the compound (in concentrations 10(-4) - 10(-5) M) caused a reduction in spike overshoot (15-20% of total amplitude) and increased the lability of responses to electrical stimulation. The somatic membrane appeared to be more affected than the axonal membrane. Diffusion barriers in the ganglion probably account for the high degree of variability in drug response, such that both of the characteristic changes were observed in only about 30% of the tests. This is the first report to describe effects of delta9-THC on invertebrate neurones. The results indicate that delta9-THC causes a depression in nerve cell excitability, and these data are consistent with reported effects of THC compounds in mammals.
在对加州海兔分离的颊神经节和顶-内脏神经节进行胞内记录期间，研究了δ9-四氢大麻酚（δ9-THC）对动作电位的影响。当将该化合物（浓度为10(-4)-10(-5)M）添加到浸泡标本的盐溶液中时，会导致动作电位超射降低（占总幅度的15-20%），并增加对电刺激反应的不稳定程度。胞体膜似乎比轴突膜受影响更大。神经节中的扩散屏障可能是药物反应高度可变的原因，以至于在仅约30%的测试中观察到了这两种特征性变化。这是第一篇描述δ9-THC对无脊椎动物神经元影响的报告。结果表明，δ9-THC会导致神经细胞兴奋性降低，这些数据与已报道的THC化合物在哺乳动物中的作用一致。
