Nanninga N, Koppes L J, de Vries-Tijssen F C
Arch Microbiol. 1979 Nov;123(2):173-81. doi: 10.1007/BF00446817.
Bacillus subtilis strain Marburg was grown exponentially with a doubling time of 65 min. To follow the time course of various cell cycle events, cells were collected by agar filtration and were then classified according to length. The DNA replication cycle was determined by a quantitative analysis of radioautograms of tritiated thymidine pulse labeled cells. The DNA replication period was found to be 45 min. This period is preceded and followed by periods without DNA synthesis of about 10 min. The morphology and segregation of nucleoplasmic bodies was studied in thin sections. B. subtilis contains two sets of genomes. DNA replication and DNA segregation seem to go hand in hand and DNA segregation is completed shortly after termination of DNA replication. Cell division and cell separation were investigated in whole mount preparations (agar filtration) and in thin sections. Cell division starts about 20 min after cell birth; cell separation starts at about 45 min and before completion of the septum.
枯草芽孢杆菌马尔堡菌株以65分钟的倍增时间呈指数生长。为了追踪各种细胞周期事件的时间进程，通过琼脂过滤收集细胞，然后根据长度进行分类。通过对氚标记胸腺嘧啶脉冲标记细胞的放射自显影片进行定量分析来确定DNA复制周期。发现DNA复制期为45分钟。在此期间之前和之后各有约10分钟的无DNA合成期。在超薄切片中研究了核质体的形态和分离。枯草芽孢杆菌含有两套基因组。DNA复制和DNA分离似乎是同步进行的，并且DNA分离在DNA复制终止后不久完成。在整装标本（琼脂过滤）和超薄切片中研究了细胞分裂和细胞分离。细胞分裂在细胞出生后约20分钟开始；细胞分离在约45分钟开始且在隔膜完成之前。
