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枸杞果实中免疫活性成分的分离、纯化及理化性质

[Isolation, purification and physico-chemical properties of immunoactive constituents from the fruit of Lycium barbarum L].

作者信息

Huang L, Lin Y, Tian G, Ji G

机构信息

Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai 200032.

出版信息

Yao Xue Xue Bao. 1998 Jul;33(7):512-6.

PMID:12016884
Abstract

Three glycoconjugates, LbGp3, LbGp4 and LbGp5, were isolated from the fruit of Lycium barbarum L. Molecular weights of LbGp3, LbGp4 and LbGp5 were 9.25 x 10(4), 21.48 x 10(4) and 2.37 x 10(4), respectively. Carbohydrate contents of LbGp3, LbGP4 and LbGp5 were 93.6%, 85.6%, 8.6%, respectively. LbGp3 was composed of Ara and Gal in a molar ratio of 1:1. LbGp4 was composed of Ara, Gal, Rha and Glc in a molar ratio of 1.5:2.5:0.43:0.23. LbGp5 was composed of Rha, Ara, Xyl, Gal, Man and Glc in a molar ratio of 0.33:0.52:0.42:0.94:0.85:1. Elemental analysis of N contents: LbGp3 0.83%, LbGp4 1.72%, LbGp5 9.58%. The linkage between the glycan and protein may be of O-linkage in LbGp4.

摘要

从宁夏枸杞果实中分离出三种糖缀合物,即枸杞糖蛋白3(LbGp3)、枸杞糖蛋白4(LbGp4)和枸杞糖蛋白5(LbGp5)。LbGp3、LbGp4和LbGp5的分子量分别为9.25×10⁴、21.48×10⁴和2.37×10⁴。LbGp3、LbGp4和LbGp5的碳水化合物含量分别为93.6%、85.6%、8.6%。LbGp3由阿拉伯糖(Ara)和半乳糖(Gal)以1:1的摩尔比组成。LbGp4由阿拉伯糖、半乳糖、鼠李糖(Rha)和葡萄糖(Glc)以1.5:2.5:0.43:0.23的摩尔比组成。LbGp5由鼠李糖、阿拉伯糖、木糖(Xyl)、半乳糖、甘露糖(Man)和葡萄糖以0.33:0.52:0.42:0.94:0.85:1的摩尔比组成。氮含量的元素分析结果为:LbGp3含0.83%,LbGp4含1.72%,LbGp5含9.58%。在LbGp4中,聚糖与蛋白质之间的连接可能为O-连接。

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