Titus R G, Weiser R S
J Infect Dis. 1979 Dec;140(6):904-13. doi: 10.1093/infdis/140.6.904.
A preparation of immunoglobulin G isolated from a pool of immune sera derived from rabbits with long-term syphilis was shown to possess a high degree of purity as judged by immunodiffusion and protein electrophoresis. The antitreponemal power of the preparation of immunoglobulin G and that of the immune serum pool from which it was derived were found to be equivalent in both the skin protection and the systemic protection test. The observation that neither normal serum nor a pool of serum derived from animals "immunized" with Salmonella typhi, Escherichia coli, Streptococcus pyogenes, or zymosan was protective indicates that the protective power of the immune serum studied was due to specific antibodies residing principally, if not entirely, in the immunoglobulin G fraction of the serum.
从患有长期梅毒的兔免疫血清池中分离出的免疫球蛋白G制剂，经免疫扩散和蛋白质电泳判断，显示具有高度纯度。在皮肤保护试验和全身保护试验中，发现该免疫球蛋白G制剂及其来源的免疫血清池的抗梅毒螺旋体能力相当。观察到正常血清以及用伤寒沙门氏菌、大肠杆菌、化脓性链球菌或酵母聚糖“免疫”的动物血清池均无保护作用，这表明所研究的免疫血清的保护能力主要（如果不是完全）归因于血清免疫球蛋白G组分中的特异性抗体。
