Chiu Tony T Y, Rogers Michael S, Law Eric L K, Briton-Jones Christine M, Cheung L P, Haines Christopher J
Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Hum Reprod. 2002 Jun;17(6):1591-6. doi: 10.1093/humrep/17.6.1591.
The follicular microenvironment is an important determinant of oocyte development. The aim of this study was to examine whether the myo-inositol (MI) content in human follicular fluid (FF) was associated with better oocyte quality.
A total of 53 patients treated with IVF was recruited to a prospective observational study. FF and serum samples collected were divided into two groups: group A consisted of FF associated with matured and fertilized oocytes, whilst group B was from follicles with immature and unfertilized oocytes.
Patient's age, total ampoules of HMG used, days of stimulation, basal levels of FSH, estradiol (E(2)) levels on the day of HCG, and serum MI content were not significantly different between the two groups. FF volume and its MI content were significantly higher in group A compared with group B (P < 0.05). The levels of MI in FF were positively correlated with the amount of E(2) in their corresponding FF samples and also correlated with embryo quality.
We propose that higher concentrations of MI and E(2) in human FF appear to play a role in follicular maturity and provide a marker of good quality oocytes.
卵泡微环境是卵母细胞发育的重要决定因素。本研究的目的是探讨人卵泡液（FF）中的肌醇（MI）含量是否与更好的卵母细胞质量相关。
共招募53例接受体外受精治疗的患者进行前瞻性观察研究。收集的FF和血清样本分为两组：A组由与成熟且受精的卵母细胞相关的FF组成，而B组来自未成熟且未受精的卵泡。
两组患者的年龄、使用的HMG总安瓿数、刺激天数、FSH基础水平、HCG当天的雌二醇（E₂）水平以及血清MI含量无显著差异。A组的FF体积及其MI含量显著高于B组（P < 0.05）。FF中的MI水平与其相应FF样本中的E₂量呈正相关，也与胚胎质量相关。
我们提出，人FF中较高浓度的MI和E₂似乎在卵泡成熟中起作用，并提供优质卵母细胞的标志物。
