群居绿藻美丽团藻中的突变体富集现象。
Mutant enrichment in the colonial alga, Eudorina elegans.
作者信息
Toby A L, Kemp C L
出版信息
Genetics. 1975 Oct;81(2):243-51. doi: 10.1093/genetics/81.2.243.
Abstract
An enrichment procedure has been developed that results in at least a 200 X increase in mutation frequency in the colonial alga, Eudorina elegans. A period of nitrogen starvation followed by treatment with 8-azaguanine results in the death of wild-type cells and the maintenance of mutants. N'-nitro-N-nitro-soguanidine-induced acetate, p-aminobenzoic acid and reduced nitrogen requiring mutants have been isolated by this procedure.
摘要
已经开发出一种富集程序，该程序可使群体藻类秀丽隐藻的突变频率至少提高200倍。一段氮饥饿期后用8-氮杂鸟嘌呤处理会导致野生型细胞死亡并使突变体得以维持。通过该程序已分离出由N'-硝基-N-亚硝基胍诱导的乙酸盐、对氨基苯甲酸和需要还原氮的突变体。
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1
Effect of 8-azaguanine on growth and viability of Bacillus megaterium.
J Bacteriol. 1962 Jan;83(1):27-34. doi: 10.1128/jb.83.1.27-34.1962.
2
Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium.
J Bacteriol. 1962 Jan;83(1):35-7. doi: 10.1128/jb.83.1.35-37.1962.
3
A replica plating technique for the isolation of nutritionally exacting mutants of a filamentous fungus (Aspergillus nidulans).
J Gen Microbiol. 1959 Jun;20(3):540-8. doi: 10.1099/00221287-20-3-540.
4
Selection and isolation of auxotrophic yeast mutants with the aid of antibiotics.
J Bacteriol. 1959 Jun;77(6):673-7. doi: 10.1128/jb.77.6.673-677.1959.
5
Selection of auxotrophic bacterial mutants by tritium-labeled thymidine.
Science. 1959 Mar 27;129(3352):838-9. doi: 10.1126/science.129.3352.838.
6
Incorporation of 8-azaguanine and growth inhibition Bacillus cereus.
J Biol Chem. 1957 Mar;225(1):137-50.
7
An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.
Nature. 1966 Jul 9;211(5045):206-7. doi: 10.1038/211206a0.
8
Synchronization of mammalian cells with tritiated thymidine.
Science. 1966 Feb 11;151(3711):691-4. doi: 10.1126/science.151.3711.691.
9
The selection of auxotrophs of Penicillium chrysognystatin.
Genet Res. 1968 Jun;11(3):327-30. doi: 10.1017/s0016672300011514.
10
Selective action of amphotericin B in the isolation of fermentation mutants of yeast.
J Bacteriol. 1968 Jul;96(1):283-4. doi: 10.1128/jb.96.1.283-284.1968.
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