Kraffe Edouard, Soudant Philippe, Marty Yanic, Kervarec Nelly, Jehan Philippe
Unité Mixte CNRS 6521, Université de Bretagne Occidentale, Brest, France.
Lipids. 2002 May;37(5):507-14. doi: 10.1007/s11745-002-0925-z.
Separation of phospholipid classes in lipid extracts from the scallop Pecten maximus, the Pacific oyster Crassostrea gigas, and the blue mussel Mytilus edulis was conducted using HPLC. An isolated polar lipid fraction was found to contain a very high level of DHA, up to 80 mol% of the total FA. MS with electrospray ionization in the positive-ion mode, tandem MS (MS-MS) and multidimensional NMR spectroscopy were used to analyze the detailed chemical structure of this polar lipid fraction. The isolated fraction contained exclusively cardiolipin (CL) molecules, predominantly in a form with four docosahexaenoyl chains (Do4CL). To the best of our knowledge, this is the first time that such a CL form has been analytically characterized and described in these three bivalve species. This tetradocosahexaenoic CL is presumed to reflect a specific adaptation in bivalves that enhances the structural and functional mechanisms of biomembranes in response to variations in environmental conditions (temperature, salinity, emersion).
利用高效液相色谱法（HPLC）对扇贝（Pecten maximus）、太平洋牡蛎（Crassostrea gigas）和蓝贻贝（Mytilus edulis）脂质提取物中的磷脂类别进行了分离。发现一个分离出的极性脂质部分含有非常高含量的二十二碳六烯酸（DHA），占总脂肪酸（FA）的80摩尔%。采用正离子模式的电喷雾电离质谱（MS）、串联质谱（MS-MS）和多维核磁共振光谱对该极性脂质部分的详细化学结构进行了分析。分离出的部分仅含有心磷脂（CL）分子，主要为具有四条二十二碳六烯酰链的形式（Do4CL）。据我们所知，这是首次在这三种双壳类物种中对这种CL形式进行分析表征和描述。这种四二十二碳六烯酸心磷脂被认为反映了双壳类动物的一种特殊适应性，可增强生物膜在应对环境条件（温度、盐度、暴露）变化时的结构和功能机制。
