Liao Haihong, Mutvei Harry, Hammarström Lars, Wurtz Tilmann, Li Jianguo
Department of Clinical Research, Center for Oral Biology, Karolinska Institutet, Sweden.
Biomaterials. 2002 Jul;23(13):2693-701. doi: 10.1016/s0142-9612(01)00421-5.
The interface of bone and aragonite nacre (Margaritifera, fresh water pearl mussel) was studied by in situ hybridization and a tartrate-resistant acid phosphatase (TRAP) histochemical assay. Columnar implants were inserted into rat femora for 4, 7, 14, 28 and 56 days. In medullary region, a burst of transient bone formation was observed, which propagated from the periphery towards the nacre implant. A fused interface of bone and nacre was observed at 14 days. Later, the new medullary bone was resorbed and bone marrow was re-established while a thin layer of bone tissue remained covering the implant surface. Expressions of collagen alpha1(I), osteocalcin, osteopontin mRNAs and TRAP in the surrounding tissue were monitored. Correlated with the histology events, a strong transient induction of collagen alpha1(I) and osteocalcin mRNAs as well as TRAP expression, exhibiting a peak signal intensity on day 7 and subsequent down-regulation after day 14 was observed. Osteopontin mRNA, in contrast, was expressed continuously. The degrading nacre surface appeared in direct contact with macrophages and multinucleated giant cells at both days 14 and 28. These cells expressed osteopontin mRNA intensively and some TRAP enzyme activity occasionally.
通过原位杂交和抗酒石酸酸性磷酸酶（TRAP）组织化学分析，研究了骨与文石珍珠层（淡水珍珠蚌）的界面。将柱状植入物插入大鼠股骨4、7、14、28和56天。在髓质区域，观察到一阵短暂的骨形成，其从周边向珍珠层植入物传播。在14天时观察到骨与珍珠层的融合界面。后来，新的髓质骨被吸收，骨髓重新建立，而一层薄的骨组织仍覆盖在植入物表面。监测周围组织中胶原蛋白α1（I）、骨钙素、骨桥蛋白mRNA和TRAP的表达。与组织学事件相关，观察到胶原蛋白α1（I）和骨钙素mRNA以及TRAP表达有强烈的短暂诱导，在第7天显示出峰值信号强度，在第14天后随后下调。相比之下，骨桥蛋白mRNA持续表达。在第14天和第28天，降解的珍珠层表面似乎与巨噬细胞和多核巨细胞直接接触。这些细胞强烈表达骨桥蛋白mRNA，偶尔有一些TRAP酶活性。
