酿酒酵母中的需氧和厌氧NAD⁺代谢。

Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae.

作者信息

Panozzo Cristina, Nawara Magdalena, Suski Catherine, Kucharczyka Roza, Skoneczny Marek, Bécam Anne Marie, Rytka Joanna, Herbert Christopher J

机构信息

Centre de Génétique Moléculaire, Laboratoire propre du CNRS, associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.

出版信息

FEBS Lett. 2002 Apr 24;517(1-3):97-102. doi: 10.1016/s0014-5793(02)02585-1.

DOI:10.1016/s0014-5793(02)02585-1

PMID:12062417

Abstract

In Saccharomyces cerevisiae the nicotinic acid moiety of NAD+ can be synthesized from tryptophan using the kynurenine pathway or incorporated directly using nicotinate phosphoribosyl transferase (NPT1). We have identified the genes that encode the enzymes of the kynurenine pathway and for BNA5 (YLR231c) and BNA6 (YFR047c) confirmed that they encode kynureninase and quinolinate phosphoribosyl transferase respectively. We show that deletion of genes encoding kynurenine pathway enzymes are co-lethal with the Deltanpt1, demonstrating that no other pathway for the synthesis of nicotinic acid exists in S. cerevisiae. Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph.

摘要

在酿酒酵母中，NAD⁺的烟酸部分可以通过犬尿氨酸途径由色氨酸合成，或者直接利用烟酸磷酸核糖基转移酶（NPT1）掺入。我们已经鉴定出编码犬尿氨酸途径中酶的基因，对于BNA5（YLR231c）和BNA6（YFR047c），证实它们分别编码犬尿氨酸酶和喹啉酸磷酸核糖基转移酶。我们表明，编码犬尿氨酸途径酶的基因缺失与Δnpt1是共致死的，这表明酿酒酵母中不存在其他合成烟酸的途径。此外，我们表明在厌氧条件下酿酒酵母是烟酸营养缺陷型。