Bostan M, Brasoveanu L I, Livescu A, Manda G, Neagu M, Iordachescu D
Center of Immunology, Institute of Virology St. S. Nicolau, Bucharest, Romania.
J Cell Mol Med. 2001 Apr-Jun;5(2):188-94. doi: 10.1111/j.1582-4934.2001.tb00152.x.
Neutrophil infiltration in the synovia is an important feature of the local inflammatory process associated with rheumatoid arthritis. The present study is focused on the effects exerted in vitro by the synovial fluid versus serum on the respiratory burst of granulocytes isolated either from blood or synovial fluid of rheumatoid arthritis patients. The respiratory burst was evaluated as superoxide anion release, by lucigenin-amplified chemiluminescence. Our data show that the respiratory burst of granulocytes isolated from rheumatoid arthritis patients might trigger a significant oxidative stress both in periphery and the inflamed joint. These cells show no pathological pattern when activated in vitro by the chemotactic peptide fMLP, heterologous synovial fluid or serum. Acellular synovial fluid amplifies the superoxide anion release induced by fMLP more than the corresponding serum, indicating that a bacterial infection in the joint might enhance the oxidative damage in the inflamed synovium.
滑膜中的中性粒细胞浸润是类风湿关节炎相关局部炎症过程的一个重要特征。本研究聚焦于类风湿关节炎患者血液或滑膜液中分离出的粒细胞，比较滑膜液与血清在体外对其呼吸爆发的影响。通过光泽精增强化学发光法，将呼吸爆发评估为超氧阴离子释放。我们的数据表明，从类风湿关节炎患者分离出的粒细胞的呼吸爆发可能在外周和炎症关节中引发显著的氧化应激。当这些细胞在体外被趋化肽fMLP、异源滑膜液或血清激活时，未显示出病理模式。无细胞滑膜液比相应血清更能增强fMLP诱导的超氧阴离子释放，表明关节中的细菌感染可能会加剧炎症滑膜中的氧化损伤。
