Zheng Haiyan, Bednarek Sebastian Y, Sanderfoot Anton A, Alonso Jose, Ecker Joseph R, Raikhel Natasha V
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544, USA.
Plant Physiol. 2002 Jun;129(2):530-9. doi: 10.1104/pp.003970.
SNAREs are important components of the vesicle trafficking machinery in eukaryotic cells. In plants, SNAREs have been found to play a variety of roles in the development and physiology of the whole organism. Here, we describe the identification and characterization of a novel plant-specific SNARE, NPSN11, a member of a closely related small gene family in Arabidopsis. NSPN11 is highly expressed in actively dividing cells. In a subcellular fractionation experiment, NSPN11 cofractionates with the cytokinesis-specific syntaxin, KNOLLE, which is required for the formation of the cell plate. By immunofluorescence microscopy, NSPN11 was localized to the cell plate in dividing cells. Consistent with the localization studies, NSPN11 was found to interact with KNOLLE. Our results suggest that NPSN11 is another component of the membrane trafficking and fusion machinery involved in cell plate formation.
SNARE蛋白是真核细胞中囊泡运输机制的重要组成部分。在植物中，已发现SNARE蛋白在整个生物体的发育和生理过程中发挥多种作用。在此，我们描述了一种新型植物特异性SNARE蛋白NPSN11的鉴定和特性，它是拟南芥中一个密切相关的小基因家族的成员。NSPN11在活跃分裂的细胞中高度表达。在亚细胞分级分离实验中，NSPN11与细胞分裂特异性 syntaxin蛋白KNOLLE共分级，KNOLLE是细胞板形成所必需的。通过免疫荧光显微镜观察，NSPN11定位于分裂细胞的细胞板上。与定位研究一致，发现NSPN11与KNOLLE相互作用。我们的结果表明，NPSN11是参与细胞板形成的膜运输和融合机制的另一个组成部分。
