Matsuda Shojiro, Se Naomi, Iwata Hiroo, Ikada Yoshito
Institute for Frontier Medical Sciences, Kyoto University, Japan.
Biomaterials. 2002 Jul;23(14):2901-8. doi: 10.1016/s0142-9612(01)00418-5.
Among five kinds of rat adhesion models tested, the following model was selected. The epigastric vein 2.5 cm from the midline of the abdomen was cut by sharp scissors, and the lateral side of the cut epigastric vein was ligated using a 3-0 silk suture. This model could be easily prepared and gave a rate of adhesion formation of 90%, which was useful for screening antiadhesive materials. For the kinetic study of tissue adhesion in this model, an injured site was covered with a non-degradable poly(vinyl alcohol) (PVA) film. The incidence rate of adhesion was 18%, when the PVA film covered the injured site for 2 days. This suggests that an antiadhesive barrier should cover the injured site for at least 2 days. The antiadhesion efficacy of cross-linked gelatin films were evaluated using this adhesion model. The UV cross-linked gelatin film which was designed to exist for 2 days but to disappear at day 3 in the rat abdominal cavity showed the highest antiadhesion efficacy.
在测试的五种大鼠粘连模型中，选择了以下模型。用锋利的剪刀在距腹部中线2.5厘米处剪断腹壁静脉，并用3-0丝线结扎剪断的腹壁静脉的外侧。该模型易于制备，粘连形成率为90%，可用于筛选抗粘连材料。为了对该模型中的组织粘连进行动力学研究，在损伤部位覆盖一层不可降解的聚乙烯醇（PVA）薄膜。当PVA薄膜覆盖损伤部位2天时，粘连发生率为18%。这表明抗粘连屏障应覆盖损伤部位至少2天。使用该粘连模型评估交联明胶薄膜的抗粘连效果。设计在大鼠腹腔中存在2天但在第3天消失的紫外线交联明胶薄膜显示出最高的抗粘连效果。
