Gip Phung, Hagiwara Grace, Ruby Norman F, Heller H Craig
Department of Biological Sciences, Stanford University, 371 Serra Mall, Stanford, CA 94305-5020, USA.
Am J Physiol Regul Integr Comp Physiol. 2002 Jul;283(1):R54-9. doi: 10.1152/ajpregu.00735.2001.
We tested whether brain glycogen reserves were depleted by sleep deprivation (SD) in Long-Evans rats 20-59 days old. Animals were sleep deprived beginning at lights on and then immediately killed by microwave irradiation. Glycogen and glucose levels were measured by a fluorescence enzymatic assay. In all age groups, SD reduced cerebellar glycogen levels by an average of 26% after 6 h of SD. No changes were observed in the cortex after 6 h of SD, but in the oldest animals, 12 h of SD increased cortical glycogen levels. There was a developmental increase in basal glycogen levels in both the cortex and cerebellum that peaked at 34 days and declined thereafter. Robust differences in cortical and cerebellar glycogen levels in response to enforced waking may reflect regional differences in energy utilization and regulation during wakefulness. These results show that brain glycogen reserves are sensitive to SD.
我们测试了20至59日龄的Long-Evans大鼠的脑糖原储备是否会因睡眠剥夺（SD）而耗尽。动物在光照开始时被剥夺睡眠，然后立即通过微波辐射处死。通过荧光酶法测量糖原和葡萄糖水平。在所有年龄组中，睡眠剥夺6小时后，小脑糖原水平平均降低了26%。睡眠剥夺6小时后，皮层未观察到变化，但在最年长的动物中，睡眠剥夺12小时会增加皮层糖原水平。皮层和小脑中的基础糖原水平在发育过程中有所增加，在34天时达到峰值，此后下降。皮层和小脑糖原水平对强制清醒的强烈差异可能反映了清醒期间能量利用和调节的区域差异。这些结果表明，脑糖原储备对睡眠剥夺敏感。
