Lee Sang Bok, Mitchell David T, Trofin Lacramioara, Nevanen Tarja K, Söderlund Hans, Martin Charles R
Department of Chemistry and Center for Research at the Bio/Nano Interface, University of Florida, Gainesville, FL 32611-7200, USA.
Science. 2002 Jun 21;296(5576):2198-200. doi: 10.1126/science.1071396.
Synthetic bio-nanotube membranes were developed and used to separate two enantiomers of a chiral drug. These membranes are based on alumina films that have cylindrical pores with monodisperse nanoscopic diameters (for example, 20 nanometers). Silica nanotubes were chemically synthesized within the pores of these films, and an antibody that selectively binds one of the enantiomers of the drug was attached to the inner walls of the silica nanotubes. These membranes selectively transport the enantiomer that specifically binds to the antibody, relative to the enantiomer that has lower affinity for the antibody. The solvent dimethyl sulfoxide was used to tune the antibody binding affinity. The enantiomeric selectivity coefficient increases as the inside diameter of the silica nanotubes decreases.
合成生物纳米管膜被开发出来并用于分离一种手性药物的两种对映体。这些膜基于具有单分散纳米级直径（例如20纳米）的圆柱形孔的氧化铝膜。在这些膜的孔内化学合成二氧化硅纳米管，并将选择性结合该药物对映体之一的抗体附着到二氧化硅纳米管的内壁上。相对于对抗体亲和力较低的对映体，这些膜选择性地转运与抗体特异性结合的对映体。使用溶剂二甲基亚砜来调节抗体结合亲和力。随着二氧化硅纳米管内径的减小，对映体选择性系数增加。
