透明质酸对大鼠眼压的影响。

Effect of hyaluronic acid on intraocular pressure in rats.

作者信息

Benozzi Jorge, Nahum Lucio P, Campanelli Julieta L, Rosenstein Ruth E

机构信息

Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department of Human Biochemistry, Faculty of Medicine, University of Buenos Aires-Paraguay 2155, 1121 Buenos Aires, Argentina.

出版信息

Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2196-200.

PMID:12091416

Abstract

PURPOSE

To study the effect of acute or chronic intracameral injection of hyaluronic acid on intraocular pressure (IOP) in rats.

METHODS

Acute or chronic injections of hyaluronic acid were performed unilaterally in the rat eye's anterior chamber, whereas the contralateral eye was injected with saline solution. IOP was assessed daily or weekly by a tonometer in conscious rats. IOP was also assessed in both experimental groups at several intervals during the light-dark cycle.

RESULTS

A single injection of hyaluronic acid induced an increase of IOP that lasted for 8 days (P < 0.01), whereas its chronic administration during 9 weeks induced a significant and sustained increase in IOP, compared with the eye injected with vehicle (P < 0.01). This hyaluronic acid-induced hypertension was significantly decreased by the application of 1 drop of brimonidine (0.2%), latanoprost (0.005%), or timolol (0.5%). Significant daily variations of IOP were observed in both control and hyaluronic acid-injected eyes, peaking during the dark phase (P < 0.001, ANOVA).

CONCLUSIONS

These results suggest that the intracameral administration of hyaluronic acid could be a model of ocular hypertension in rats.

摘要

目的

研究急性或慢性前房内注射透明质酸对大鼠眼压（IOP）的影响。

方法

在大鼠眼的前房单侧进行急性或慢性透明质酸注射，而对侧眼注射盐溶液。通过眼压计每日或每周对清醒大鼠的眼压进行评估。在明暗周期的几个时间点也对两个实验组的眼压进行评估。

结果

单次注射透明质酸可使眼压升高并持续8天（P < 0.01），而与注射赋形剂的眼相比，在9周内慢性给药可使眼压显著且持续升高（P < 0.01）。应用1滴溴莫尼定（0.2%）、拉坦前列素（0.005%）或噻吗洛尔（0.5%）可使透明质酸诱导的高血压显著降低。在对照眼和注射透明质酸的眼中均观察到眼压的显著每日变化，在暗期达到峰值（P < 0.001，方差分析）。