Hamada S, Mizuno J, Kotani S
Microbios. 1979;25(101-102):155-66.
Cell-free supernatant of Streptococcus mutans cultures was concentrated by 50% ammonium sulphate precipitation to 1/50 of the original culture volume. Most extracellular glucosyltransferase activity was detected in the supernatant. Furthermore, the supernatant reacted with serotype specific antiserum, showing immunological identities with the serotype specific polysaccharide antigen obtained from whole cells by hot saline or NaOH extraction in double diffusion tests. Immunoelectrophoretic analysis also revealed that the supernatant antigen possessed significant quantities of lipid-bound and free glycerol teichoic acid antigens. The lipoteichoic acid (LTA) was obtained from whole cells by phenol-water extraction. The lipid-teichoic acid linkage was easily split by mild acid or alkaline hydrolysis. The supernatant and phenol antigens spontaneously sensitized sheep red blood cells. These antigens were adsorbed to hydroxyapatite powder. The extracellular LTA and glucosyltransferase were found to be closely associated and difficult to separate chromatographically. In vivo significance of these properties of LTA and GTase is discussed in terms of the ecology of S. mutans and other oral streptococci.
变形链球菌培养物的无细胞上清液通过50%硫酸铵沉淀浓缩至原始培养物体积的1/50。大部分细胞外葡糖基转移酶活性在上清液中检测到。此外，该上清液与血清型特异性抗血清发生反应，在双向扩散试验中显示出与通过热盐水或氢氧化钠从全细胞中提取的血清型特异性多糖抗原具有免疫同一性。免疫电泳分析还表明，上清液抗原含有大量的脂质结合型和游离甘油磷壁酸抗原。脂磷壁酸（LTA）通过酚-水提取法从全细胞中获得。脂质-磷壁酸连接很容易被弱酸或碱水解断裂。上清液和酚抗原能自发致敏绵羊红细胞。这些抗原被吸附到羟基磷灰石粉末上。发现细胞外LTA和葡糖基转移酶紧密相关，难以通过色谱法分离。从变形链球菌和其他口腔链球菌的生态学角度讨论了LTA和GTase这些特性在体内的意义。
