Hamada S, Tai S, Slade H D
Infect Immun. 1976 Oct;14(4):903-10. doi: 10.1128/iai.14.4.903-910.1976.
Hot saline extracts of Streptococcus mutans have been shown to contain antigenic substances which occasionally react nonspecifically with some antisera against whole cells of various serological groups and types of streptococci. Chromatography of the extract of S. mutans strain MT703 (serotype e) on a diethylaminoethyl-Sephadex A-25 column gave two principal antigens. One antigen was eluted without adsorption to the resin and was identified as the serotype-specific polysaccharide. The other antigen, which contained a large quantity of phosphorus, was absorbed to and released from the resin by gradient elution. It was reactive against the antisera specific for polyglycerophosphate (PGP) from group A Streptococcus pyogenes and/or S. mutans strain Ingbritt (type c). The PGP antigen was further purified by gel filtration with Sephadex G-75. Two peaks, PGP-1, and PGP-2, were obtained. Each possessed the same antigenic specificity to anti-PGP serum as shown by immunodiffusion. Chemical analyses revealed that the molar ratio of phosphorus to glycerol in both was about 1:1, although the protein content between the two was significantly different. PGP antigen was found to be widely distributed in hot saline extracts from various gram-positive bacteria, with a few exceptions. However, all gram-negative bacteria examined were free of PGP. The PGP in the hot saline extracts of various gram-positive bacteria possessed an essentially identical antigenic specificity. The addition of diethylaminoethyl-Sephadex A-25 resin to hot saline extracts successfully removed the cross-reacting PGP antigen. After adsorption of the extract from S. mutans, the supernatant contained only type-specific polysaccharide antigen, except type b, in which both type b-specific polysaccharide and PGP antigens were absorbed with the resin. This simple procedure should be useful for the removal of the PGP-type teichoic acid from antigen extracts of bacteria that contain uncharged polysaccharides.
变形链球菌的热盐水提取物已被证明含有抗原性物质，这些物质偶尔会与一些针对各种血清学组和类型链球菌全细胞的抗血清发生非特异性反应。在二乙氨基乙基 - 葡聚糖A - 25柱上对变形链球菌MT703菌株（血清型e）的提取物进行色谱分析，得到了两种主要抗原。一种抗原未被树脂吸附而被洗脱，被鉴定为血清型特异性多糖。另一种含有大量磷的抗原，通过梯度洗脱被树脂吸附并从树脂上释放。它与化脓性链球菌A组和/或变形链球菌Ingbritt菌株（c型）的聚甘油磷酸（PGP）特异性抗血清发生反应。PGP抗原通过用葡聚糖G - 75进行凝胶过滤进一步纯化。得到了两个峰，PGP - 1和PGP - 2。免疫扩散显示，它们对抗PGP血清具有相同的抗原特异性。化学分析表明，两者中磷与甘油的摩尔比约为1:1，尽管两者之间的蛋白质含量有显著差异。发现PGP抗原广泛分布于各种革兰氏阳性菌的热盐水提取物中，少数例外。然而，所有检测的革兰氏阴性菌均不含PGP。各种革兰氏阳性菌热盐水提取物中的PGP具有基本相同的抗原特异性。向热盐水提取物中加入二乙氨基乙基 - 葡聚糖A - 25树脂成功去除了交叉反应的PGP抗原。从变形链球菌提取物吸附后，除b型外，上清液仅含有型特异性多糖抗原，在b型中，b型特异性多糖和PGP抗原均被树脂吸附。这个简单的程序对于从含有不带电荷多糖的细菌抗原提取物中去除PGP型磷壁酸应该是有用的。
