Silwood C J L, Lynch E, Claxson A W D, Grootveld M C
Medical Unit, St. Bartholomews and the Royal London School of Medicine and Dentistry, London E1 1BB, UK.
J Dent Res. 2002 Jun;81(6):422-7. doi: 10.1177/154405910208100613.
We have explored the ability of high-resolution NMR techniques to (1) index salivary biomolecules and (2) provide valuable data regarding intra- and inter-subject variability in the concentrations of a series of components readily determinable by this technique (organic acids and malodorous amines). Experiments were conducted on 'whole' saliva samples collected from 20 patients, either randomly during their daily activities, or, for investigations involving the quantification of salivary biomolecules, immediately after they woke in the morning throughout a three-day period. These NMR techniques permitted us to detect greater than 60 metabolites, together with agents arising from dietary, oral health care product, and pharmaceutical sources. Highly significant "between-subject" differences in the a.m. waking salivary metabolite concentrations were found for 9 out of 11 components monitored. It is concluded that NMR spectroscopy serves as a powerful technique for the multicomponent analysis of human saliva.
我们已经探索了高分辨率核磁共振技术的能力，以（1）对唾液生物分子进行索引，以及（2）提供有关一系列可通过该技术轻松测定的成分（有机酸和恶臭胺）在个体内和个体间浓度变异性的有价值数据。对从20名患者收集的“全”唾液样本进行了实验，样本要么在他们日常活动期间随机收集，要么在为期三天的时间里，在他们早上醒来后立即收集，用于涉及唾液生物分子定量的研究。这些核磁共振技术使我们能够检测到60多种代谢物，以及来自饮食、口腔护理产品和药物来源的物质。在所监测的11种成分中，有9种在上午醒来时唾液代谢物浓度存在高度显著的“个体间”差异。得出的结论是，核磁共振光谱法是一种用于人类唾液多成分分析的强大技术。
