Almer Laurel S, Shortridge Virginia D, Nilius Angela M, Beyer Jill M, Soni Niru B, Bui Mai H, Stone Gregory G, Flamm Robert K
Infectious Diseases Research, Abbott Laboratories, Abbott Park, IL, USA.
Diagn Microbiol Infect Dis. 2002 Jul;43(3):225-32. doi: 10.1016/s0732-8893(02)00394-2.
Community-acquired methicillin-resistant Staphylococcus aureus (MRSA) infections are increasing. Since most published data are on nosocomial MRSA, our goal was to identify the antimicrobial susceptibility profile and resistance mechanisms of pretreatment MRSA isolates obtained from adult subjects participating in recent clinical treatment trials of community respiratory infections. Out of 465 S. aureus isolates, 43 were identified as MRSA. Antimicrobial susceptibility testing indicated susceptibility rates to: vancomycin (100%), gentamicin (86%), clindamycin (39%), quinolones (49%), and erythromycin (12%). Among our MRSA isolates, the MLS constitutive phenotype and ermA were more prevalent than the MLS inducible phenotype and ermC. No isolates had ermB or msrA. All ciprofloxacin resistant isolates had an amino acid change in GyrA and GrlA. The relatedness of our MRSA strains was assessed by ribotyping. Our results indicate that MRSA from adult subjects with community respiratory infections have similar antimicrobial susceptibility profiles and resistance mechanisms as nosocomial MRSA, and represent a genetically diverse group.
社区获得性耐甲氧西林金黄色葡萄球菌（MRSA）感染正在增加。由于大多数已发表的数据是关于医院内MRSA的，我们的目标是确定从参与近期社区呼吸道感染临床治疗试验的成年受试者中分离出的治疗前MRSA菌株的抗菌药敏谱和耐药机制。在465株金黄色葡萄球菌分离株中，有43株被鉴定为MRSA。抗菌药敏试验表明对以下药物的敏感率为：万古霉素（100%）、庆大霉素（86%）、克林霉素（39%）、喹诺酮类（49%）和红霉素（12%）。在我们的MRSA分离株中，MLS组成型表型和ermA比MLS诱导型表型和ermC更普遍。没有分离株含有ermB或msrA。所有对环丙沙星耐药的分离株在GyrA和GrlA中都有氨基酸变化。通过核糖体分型评估了我们的MRSA菌株的相关性。我们的结果表明，来自患有社区呼吸道感染的成年受试者的MRSA与医院内MRSA具有相似的抗菌药敏谱和耐药机制，并且代表了一个基因多样化的群体。
