铜绿假单胞菌中mexAB-oprM多药外排操纵子的MexR阻遏物：影响活性的突变特征
The mexR repressor of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa: characterization of mutations compromising activity.
作者信息
Adewoye Lateef, Sutherland Ainsley, Srikumar Ramakrishnan, Poole Keith
机构信息
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
出版信息
J Bacteriol. 2002 Aug;184(15):4308-12. doi: 10.1128/JB.184.15.4308-4312.2002.
Abstract
Mutations in mexR yield a multidrug resistance phenotype in nalB mutants of Pseudomonas aeruginosa as a result of derepression of the mexAB-oprM multidrug efflux operon. MexR produced by several nalB strains carried single amino acid changes that compromised MexR stability or its ability to dimerize. Changes at residues L95 and R21, however, produced a stable MexR protein capable of dimerization and, thus, likely compromised DNA binding.
摘要
mexR基因的突变会导致铜绿假单胞菌nalB突变体出现多药耐药表型，这是由于mexAB-oprM多药外排操纵子的去阻遏作用。几个nalB菌株产生的MexR携带单个氨基酸变化，这些变化损害了MexR的稳定性或其二聚化能力。然而，L95和R21位点的变化产生了一种能够二聚化的稳定MexR蛋白，因此可能损害了DNA结合能力。
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