Lin Hong-Ru, Kuo Chun-Jung, Yang C Y, Shaw Shyh-Yu, Wu Yu-Jun
Department of Applied Chemistry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan 717.
J Biomed Mater Res. 2002;63(3):271-9. doi: 10.1002/jbm.10183.
In the present study, a mixture of ammonium-bicarbonate (NH(4)HCO(3)) and sodium-chloride (NaCl) particles was used as a porogen additive to fabricate highly macroporous biodegradable poly(lactic-co-glycolic acid) (PLGA) scaffolds. A two-step salt-leaching process was performed after the sample had become semisolidified. Compared to the standard solvent-casting/particulate-leaching (SC/PL) technique, the processing time of this approach was significantly shorter: Instead of several days, only half a day was required. In addition, the polymer/salts/solvent mixture can be easily handled and molded into scaffolds of any specific shape-for example, thin sheet, cylindrical, or bone-shaped-for special applications in tissue engineering. Our results demonstrate that these scaffolds have a highly interconnected open-pore structure as well as greater mechanical properties than those made using the standard SC/PL technique. Primary rat osteoblasts seeded into the scaffolds exhibited good seeding efficiency. The method presented here is a promising approach for fabricating scaffolds for tissue engineering applications.
在本研究中，使用碳酸氢铵（NH₄HCO₃）和氯化钠（NaCl）颗粒的混合物作为致孔剂添加剂来制备高度大孔的可生物降解聚乳酸-乙醇酸共聚物（PLGA）支架。在样品半固化后进行两步盐沥滤过程。与标准的溶剂浇铸/颗粒沥滤（SC/PL）技术相比，该方法的加工时间明显更短：不是需要几天，而仅需半天。此外，聚合物/盐/溶剂混合物可以很容易地处理并模制成任何特定形状的支架，例如薄片、圆柱形或骨形，用于组织工程中的特殊应用。我们的结果表明，这些支架具有高度互连的开放孔结构以及比使用标准SC/PL技术制备的支架更好的机械性能。接种到支架中的原代大鼠成骨细胞表现出良好的接种效率。这里介绍的方法是一种用于制造组织工程应用支架的有前途的方法。
