Hatai T, Yokozeki M, Funato N, Baba Y, Moriyama K, Ichijo H, Kuroda T
Maxillofacial Orthognathics, Tokyo Medical and Dental University, Japan.
Oral Dis. 2001 Sep;7(5):287-90. doi: 10.1034/j.1601-0825.2001.00663.x.
The mechanical force generated during tooth movement creates compressed and cell-free areas in the periodontal membrane. The way in which periodontal ligament cells disappear at the compressed area during tooth movement remains unclear. In the present study we examined whether periodontal ligament cells undergo apoptosis by mechanical stress during tooth movement using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end-labelling method (TUNEL). TUNEL-positive stainings of periodontal ligament cells began to appear at the compressed areas 12 h after tooth movement, and the number of those cells reached maximum at 24 h after tooth movement. Thereafter TUNEL-positive cells disappeared at 48 h, and direct and undermining bone resorption began at the same area 72 h after tooth movement. These results showed that compressed periodontal ligament cells were eliminated by apoptosis in the early phase of tooth movement.
牙齿移动过程中产生的机械力会在牙周膜中形成压缩区和无细胞区。在牙齿移动过程中，牙周膜细胞在压缩区消失的方式尚不清楚。在本研究中，我们使用末端脱氧核苷酸转移酶（TdT）介导的dUTP生物素缺口末端标记法（TUNEL），研究了牙齿移动过程中机械应力是否会导致牙周膜细胞发生凋亡。牙齿移动12小时后，牙周膜细胞的TUNEL阳性染色开始出现在压缩区，并且这些细胞的数量在牙齿移动24小时时达到最大值。此后，TUNEL阳性细胞在48小时时消失，并且在牙齿移动72小时后，在同一区域开始出现直接性和潜行性骨吸收。这些结果表明，在牙齿移动的早期阶段，压缩的牙周膜细胞通过凋亡被清除。
