Rigden Daniel J
Embrapa Genetic Resources and Biotechnology, Cenargen/Embrapa, S.A.I.N. Parque Rural, Final W5, Asa Norte, 70770-900, Brasília, Brazil.
FEBS Lett. 2002 Jul 17;523(1-3):17-22. doi: 10.1016/s0014-5793(02)02879-x.
Classification of glycoside hydrolases (GHs) into families, along with the structure-based grouping together of families into clans, improve our understanding of the evolution of the large natural variety of these enzymes, help rationalise experimental data and guide further studies. Here we identify triose phosphate isomerase (TIM) barrels in GH families 27, 31, 36 and 66. We further show that iterated sequence database searches provide evidence for their sharing a common evolutionary origin with GH family 13. The catalytic, nucleophilic residue common to all these families is thereby determined and candidate catalytic proton donors identified within each family.
将糖苷水解酶（GHs）分类成不同家族，以及基于结构将各家族归为不同的簇，有助于我们更好地理解这些酶在自然界中种类繁多的进化过程，使实验数据更具合理性，并为进一步研究提供指导。在此，我们在GH家族27、31、36和66中鉴定出磷酸丙糖异构酶（TIM）桶状结构。我们进一步表明，反复进行的序列数据库搜索为它们与GH家族13有着共同的进化起源提供了证据。由此确定了所有这些家族共有的催化亲核残基，并在每个家族中鉴定出候选催化质子供体。
