Beijer Lena, Thorn Jörgen, Rylander Ragnar
Department of Environmental Medicine, Göteborg University, Gothenburg, Sweden.
Mediators Inflamm. 2002 Jun;11(3):149-53. doi: 10.1080/09622935020138181.
Damp conditions indoors favour the growth of microorganisms, and these contain several agents that may cause inflammation when inhaled. Moulds contain a polyglucose in their cell wall, defined as (1-->3)-beta-D-glucan, exhibiting effects on inflammatory cells.
The aim of the present study was to evaluate whether an inhalation challenge to purified (1-->3)-beta-D-glucan (grifolan) in humans could induce effects on inflammatory markers in blood, and to evaluate whether the reactions were related to the home exposure to (1-->3)-beta-D-glucan.
Seventeen subjects in homes with high levels of airborne (1-->3)-beta-D-glucan (G-high) and 18 subjects in homes with low levels of (1-->3)-beta-D-glucan (G-low) underwent two randomised, double-blind inhalation challenges, one to (1-->3)-beta-D-glucan suspended in saline and one to saline alone. A blood sample was taken before and after the challenges, and differential cell count, granulocyte enzymes in serum and the secretion of cytokines from peripheral blood mononuclear cells (PBMC) were measured.
Inhalation challenge with (1-->3)-beta-D-glucan induced a decrease in the secretion of tumour necrosis factor alpha from endotoxin-stimulated PBMC in the G-high group as well as in the G-low group. In the G-high group, the inhalation of (1-->3)-beta-D-glucan induced an increase in blood lymphocytes that was significantly different from the saline-induced effect.
The results suggest that an inhalation challenge to (1-->3)-beta-D-glucan has an effect on inflammatory cells and this effect may be related to a chronic exposure to moulds at home.
室内潮湿环境有利于微生物生长，这些微生物含有多种吸入后可能引发炎症的物质。霉菌细胞壁中含有一种多聚糖，定义为（1→3）-β-D-葡聚糖，对炎症细胞有影响。
本研究旨在评估人体吸入纯化的（1→3）-β-D-葡聚糖（灰树花多糖）是否会对血液中的炎症标志物产生影响，并评估这些反应是否与家中接触（1→3）-β-D-葡聚糖有关。
17名居住在空气中（1→3）-β-D-葡聚糖含量高的家庭中的受试者（G高组）和18名居住在（1→3）-β-D-葡聚糖含量低的家庭中的受试者（G低组）接受了两次随机、双盲吸入激发试验，一次吸入悬浮于盐水中的（1→3）-β-D-葡聚糖，另一次仅吸入盐水。在激发试验前后采集血样，检测细胞分类计数、血清中的粒细胞酶以及外周血单核细胞（PBMC）的细胞因子分泌情况。
（1→3）-β-D-葡聚糖吸入激发试验使G高组和G低组中内毒素刺激的PBMC分泌肿瘤坏死因子α减少。在G高组中，吸入（1→3）-β-D-葡聚糖使血液淋巴细胞增加，这与盐水激发试验的效果有显著差异。
结果表明，吸入（1→3）-β-D-葡聚糖对炎症细胞有影响，且这种影响可能与家中长期接触霉菌有关。
