Kochel Tadeusz J, Watts Douglas M, Halstead Scott B, Hayes Curtis G, Espinoza Angelica, Felices Vidal, Caceda Roxana, Bautista Christian T, Montoya Ysabel, Douglas Susan, Russell Kevin L
Department of Virology, Naval Medical Research Centre Detachment, Lima, Peru.
Lancet. 2002 Jul 27;360(9329):310-2. doi: 10.1016/S0140-6736(02)09522-3.
In Iquitos, Peru, no cases of dengue haemorrhagic fever have been recorded in individuals infected with dengue-1 virus followed by American genotype dengue-2 (American dengue-2) virus. We assayed serum samples collected in Iquitos that tested positive for antibodies of monotype dengue-1 and monotype dengue-2 using a plaque reduction neutralisation test to determine their ability to neutralise the infectivity of two dengue-1 viruses, two American dengue-2 viruses, and two Asian dengue-2 viruses. Sera positive for the dengue-1 antibody neutralised dengue-1 viruses and American dengue-2 viruses much more effectively than Asian dengue-2 viruses. Neutralisation of American dengue-2 virus by sera positive for dengue-1 antibodies may account for the absence of dengue haemorrhagic fever in individuals infected with dengue-1 in 1990-91 followed by American dengue-2 virus in 1995 in Iquitos, Peru.
在秘鲁伊基托斯，感染登革热1型病毒后又感染美国基因型登革热2型（美国登革热2型）病毒的个体中，未记录到登革出血热病例。我们对在伊基托斯采集的血清样本进行了检测，这些样本通过单型登革热1型和单型登革热2型抗体检测呈阳性，我们使用蚀斑减少中和试验来确定它们中和两种登革热1型病毒、两种美国登革热2型病毒和两种亚洲登革热2型病毒感染性的能力。登革热1型抗体呈阳性的血清对登革热1型病毒和美国登革热2型病毒的中和效果比对亚洲登革热2型病毒的中和效果要有效得多。登革热1型抗体呈阳性的血清对美国登革热2型病毒的中和作用可能解释了1990 - 1991年在秘鲁伊基托斯感染登革热1型病毒，随后于1995年感染美国登革热2型病毒的个体中未出现登革出血热的原因。
