Ocheretenko L Iu, Henhin M T, Reva O D, Zelens'kyĭ H V
Ukr Biokhim Zh. 1975 May-Jun;47(3):319-22.
Soluble forms of neutral peptide-hydrolases (protaminsulphate as a substrate) of the cat cerebral hemisphere grey and white matter were investigated by the method of gel-filtration on Sephadex G-150. The results of the experiments showed that grey and white matter of the cerebral hemispheres contains different forms of soluble neutral peptide-hydrolases. The products of protaminsulphate hydrolysis were analyzed by the method of gel-filtration as well (Sephadex G-25). Under the influence of the studied enzymes of protaminsulphate hydrolysis occurs due to subsequent splitting of C-terminal residues of arginine. It means that the studied soluble neutral peptide-hydrolases of grey and white matter of the cat cerebral hemisphere may be referred to the group of exopeptidases.
采用Sephadex G - 150凝胶过滤法研究了猫脑半球灰质和白质中中性肽水解酶（以鱼精蛋白硫酸盐为底物）的可溶性形式。实验结果表明，脑半球的灰质和白质含有不同形式的可溶性中性肽水解酶。鱼精蛋白硫酸盐水解产物也采用凝胶过滤法（Sephadex G - 25）进行分析。在所研究的酶的作用下，鱼精蛋白硫酸盐的水解是由于精氨酸C末端残基的后续裂解而发生的。这意味着所研究的猫脑半球灰质和白质中的可溶性中性肽水解酶可能属于外肽酶组。
