核糖体蛋白介导丙型肝炎病毒内部核糖体进入位点与HeLa 40S亚基的相互作用。
Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction.
作者信息
Otto Geoff A, Lukavsky Peter J, Lancaster Alissa M, Sarnow Peter, Puglisi Joseph D
机构信息
Department of Structural Biology, Stanford University School of Medicine, California 94305-5126, USA.
出版信息
RNA. 2002 Jul;8(7):913-23. doi: 10.1017/s1355838202022057.
Abstract
Translation of the hepatitis C virus genomic RNA is mediated by an internal ribosome entry site (IRES). The 330-nt IRES RNA forms a binary complex with the small 40S ribosomal subunit as a first step in translation initiation. Here chemical probing and 4-thiouridine-mediated crosslinking are used to characterize the interaction of the HCV IRES with the HeLa 40S subunit. No IRES-18S rRNA contacts were detected, but several specific crosslinks to 40S ribosomal proteins were observed. The identity of the crosslinked proteins agrees well with available structural information and provides new insights into HCV IRES function. The protein-rich surface of the 40S subunit thus mediates the IRES-ribosome interaction.
摘要
丙型肝炎病毒基因组RNA的翻译由内部核糖体进入位点（IRES）介导。330个核苷酸的IRES RNA与小40S核糖体亚基形成二元复合物，作为翻译起始的第一步。在此，化学探针和4-硫尿苷介导的交联被用于表征HCV IRES与HeLa 40S亚基的相互作用。未检测到IRES与18S rRNA的接触，但观察到与40S核糖体蛋白的几个特异性交联。交联蛋白的身份与现有的结构信息高度吻合，并为HCV IRES功能提供了新的见解。因此，40S亚基富含蛋白质的表面介导了IRES与核糖体的相互作用。
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