Baldwin Richard P, Roussel Thomas J, Crain Mark M, Bathlagunda Vijay, Jackson Douglas J, Gullapalli Jayadeep, Conklin John A, Pai Rekha, Naber John F, Walsh Kevin M, Keynton Robert S
Department of Chemistry, University of Louisville, Kentucky 40292, USA.
Anal Chem. 2002 Aug 1;74(15):3690-7. doi: 10.1021/ac011188n.
Microfabricated lab-on-a-chip devices employing a fully integrated electrochemical (EC) detection system have been developed and evaluated. Both capillary electrophoresis (CE) channels and all CE/EC electrodes were incorporated directly onto glass substrates via traditional microfabrication techniques, including photolithographic patterning, wet chemical etching, DC sputtering, and thermal wafer bonding. Unlike analogous CE/EC devices previously reported, no external electrodes were required, and critical electrode characteristics, including size, shape, and placement on the microchip, were established absolutely by the photolithography process. For the model analytes dopamine and catechol, detection limits in the 4-5 microM range (approximately 200 amol injected) were obtained with the Pt EC electrodes employed here, and devices gave stable analytical performance over months of usage.
采用完全集成电化学（EC）检测系统的微制造芯片实验室设备已被开发和评估。通过传统的微制造技术，包括光刻图案化、湿化学蚀刻、直流溅射和热晶圆键合，将毛细管电泳（CE）通道和所有CE/EC电极直接集成到玻璃基板上。与先前报道的类似CE/EC设备不同，不需要外部电极，并且光刻工艺绝对确定了关键电极特性，包括尺寸、形状和在微芯片上的位置。对于模型分析物多巴胺和儿茶酚，使用此处的铂EC电极获得了4-5 microM范围内的检测限（注入量约为200 amol），并且该设备在数月的使用中具有稳定的分析性能。
