Kalashnikov Maxim, Choi Wonshik, Hunter Martin, Yu Chung-Chieh, Dasari Ramachandra R, Feld Michael S
Fraunhofer Center for Manufacturing Innovation at Boston University, Brookline, Massachusetts 02446, USA.
Opt Express. 2012 Jan 16;20(2):816-26. doi: 10.1364/OE.20.000816.
We report a method of assessing the contribution of whole cell body and its nucleus to the clinically most relevant backward light scattering. We first construct an experimental system that can measure forward scattering and use the system to precisely extract the optical properties of a specimen such as the refractive index contrast, size distribution, and their density. A system that can simultaneously detect the backscattered light is installed to collect the backscattering for the same specimen. By comparing the measured backscattering spectrum with that estimated from the parameters determined by the forward scattering experiment, the contribution of cell body and nucleus to the backward light scattering is quantitatively assessed. For the HeLa cells in suspension, we found that the cell body contributes less than 10% and cell nucleus on the order of 0.1% to the total backscattering signal. Quantitative determination of the origin of backscattered light may help design a system that aims for detecting particular structure of biological tissues.
我们报告了一种评估整个细胞体及其细胞核对临床上最相关的向后光散射贡献的方法。我们首先构建一个能够测量前向散射的实验系统，并使用该系统精确提取标本的光学特性，如折射率对比度、尺寸分布及其密度。安装一个能够同时检测背向散射光的系统，以收集同一标本的背向散射光。通过将测量的背向散射光谱与根据前向散射实验确定的参数估计的光谱进行比较，定量评估细胞体和细胞核对向后光散射的贡献。对于悬浮的HeLa细胞，我们发现细胞体对总背向散射信号的贡献小于10%，而细胞核的贡献约为0.1%。背向散射光起源的定量测定可能有助于设计一种旨在检测生物组织特定结构的系统。
