Kageyama T, Ogasawara A, Fukuhara R, Narita Y, Miwa N, Kamanaka Y, Abe M, Kumazaki K, Maeda N, Suzuki J, Gotoh S, Matsubayashi K, Hashimoto C, Kato A, Matsubayashi N
Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Japan.
J Med Primatol. 2002 Jun;31(3):129-35. doi: 10.1034/j.1600-0684.2002.01034.x.
In the last three decades, several monkeys reared in outdoor/indoor-outdoor breeding colonies and cages of the Primate Research Institute, Kyoto University, died of yersiniosis caused by Yersinia pseudotuberculosis, necessitating introduction of a method to detect the bacteria rapidly and thus allow preventive measures to be undertaken. A rapid nested polymerase chain reaction (PCR) method for identification of Y. pseudotuberculosis in fecal samples and a random amplified polymorphic DNA (RAPD)-PCR approach for distinguishing between bacterial strains were therefore developed. Yersinia pseudotuberculosis isolates from monkey specimens were found to be classifiable into several types. To determine the source of infection, hundreds of fecal samples of wild rats, pigeons, and sparrows were collected from around the breeding colonies and cages, and subjected to PCR analyses. Yersinia pseudotuberculosis was detected in 1.7% of the fecal samples of wild rats. The DNA fingerprints of the bacteria revealed by RAPD-PCR were the same as that of one strain isolated from macaques, suggesting the wild rat to be a possible source of infection.
在过去三十年里，京都大学灵长类动物研究所户外/室内-户外繁殖群落及笼舍中饲养的几只猴子死于由假结核耶尔森菌引起的耶尔森菌病，因此需要引入一种快速检测该细菌的方法，从而能够采取预防措施。为此开发了一种用于鉴定粪便样本中假结核耶尔森菌的快速巢式聚合酶链反应（PCR）方法以及一种用于区分细菌菌株的随机扩增多态性DNA（RAPD）-PCR方法。从猴子标本中分离出的假结核耶尔森菌菌株可分为几种类型。为了确定感染源，从繁殖群落及笼舍周围收集了数百份野生大鼠、鸽子和麻雀的粪便样本，并进行PCR分析。在1.7%的野生大鼠粪便样本中检测到假结核耶尔森菌。RAPD-PCR显示的该细菌DNA指纹图谱与从猕猴分离出的一个菌株相同，这表明野生大鼠可能是感染源。
