一种传统疗法导致的肝静脉闭塞病：运用体外技术确证有毒吡咯里西啶生物碱为病因
Hepatic veno-occlusive disease as a result of a traditional remedy: confirmation of toxic pyrrolizidine alkaloids as the cause, using an in vitro technique.
作者信息
Zuckerman M, Steenkamp V, Stewart M J
机构信息
Department of Paediatrics, Coronation Hospital, University of Witwatersrand, Johannesburg, South Africa.
出版信息
J Clin Pathol. 2002 Sep;55(9):676-9. doi: 10.1136/jcp.55.9.676.
BACKGROUND/AIMS: A child presented with hepatic veno-occlusive disease after having been administered a short course of treatment with a traditional herbal remedy. The child subsequently died. Postmortem liver histology confirmed the diagnosis. This study aimed to investigate the hypothesis that the herbal remedy was the cause of veno-occlusive disease.
METHODS
Extracts of the traditional remedy were analysed by colorimetry and gas chromatography/mass spectrometry. Cultured hepatocytes were treated with an extract of the plant material and examined for morphological changes.
RESULTS
The screening analyses indicated the presence of toxic pyrrolizidine alkaloids, which were later confirmed by gas chromatography/mass spectrometry. The cell studies indicated dose related toxicity, with necrosis at high concentrations and apoptosis and abnormalities of the cytoskeleton at lower concentrations.
CONCLUSIONS
The simple screening techniques used allowed rapid confirmation of the presence of toxic pyrrolizidines in the remedy. The in vitro method confirmed the toxicity of herbal extracts to hepatocytes.
背景/目的：一名儿童在接受了短疗程传统草药治疗后出现肝静脉闭塞性疾病，随后死亡。尸检肝脏组织学检查确诊了该疾病。本研究旨在调查草药是导致静脉闭塞性疾病的原因这一假设。
方法
采用比色法和气相色谱/质谱法对传统草药提取物进行分析。用植物材料提取物处理培养的肝细胞，并检查其形态变化。
结果
筛选分析表明存在有毒的吡咯里西啶生物碱，随后通过气相色谱/质谱法得到证实。细胞研究表明存在剂量相关毒性，高浓度时出现坏死，低浓度时出现凋亡和细胞骨架异常。
结论
所采用的简单筛选技术能够快速确认草药中存在有毒的吡咯里西啶。体外方法证实了草药提取物对肝细胞的毒性。
Zotero 插件