Sullman S F, Zuckerman A J
Br J Exp Pathol. 1969 Aug;50(4):361-70.
The effects of heliotrine on human embryo hepatocytes in culture and on cells of a continuous cell line derived from human liver were investigated. Cell necrosis did not occur but cytological changes consisting mainly of cytoplasmic vacuolation were produced in the hepatocytes. Progressive hypertrophy of the hepatocytes and of cells of the continuous line was observed. The enlargement of the cells increased both with the concentration and the period of exposure to the alkaloid. Heliotrine inhibited the synthesis of nucleic acids and protein by all the cell types present in primary cultures of the liver. The experimental data indicated that the action of heliotrine was primarily on the synthesis of DNA but some inhibition of RNA synthesis also occurred. It is proposed that heliotrine acts mainly in the major groove of the DNA helix inhibiting the DNA polymerase but there is also an effect on the RNA polymerase in the minor groove. The mechanism of action of heliotrine could be similar to the action of some of the antibiotics, in particular aflatoxin B.
研究了天芥菜碱对培养的人胚胎肝细胞以及源自人肝脏的连续细胞系细胞的影响。未发生细胞坏死，但肝细胞出现了主要由细胞质空泡化组成的细胞学变化。观察到肝细胞和连续细胞系的细胞逐渐肥大。细胞的增大随生物碱的浓度和暴露时间而增加。天芥菜碱抑制肝脏原代培养物中所有细胞类型的核酸和蛋白质合成。实验数据表明，天芥菜碱的作用主要作用于DNA合成，但也发生了对RNA合成的一些抑制。有人提出，天芥菜碱主要作用于DNA螺旋的大沟，抑制DNA聚合酶，但对小沟中的RNA聚合酶也有影响。天芥菜碱的作用机制可能类似于某些抗生素，特别是黄曲霉毒素B的作用。
