舌蛭食管器官内的内共生细菌。
Endosymbiotic bacteria in the esophageal organ of glossiphoniid leeches.
作者信息
Kikuchi Yoshitomo, Fukatsu Takema
机构信息
Natural History Laboratory, Faculty of Science, Ibaraki University, Mito 310-8512, Japan.
出版信息
Appl Environ Microbiol. 2002 Sep;68(9):4637-41. doi: 10.1128/AEM.68.9.4637-4641.2002.
Abstract
We characterized the intracellular symbiotic bacteria of the hematophagous glossiphoniid leeches Placobdelloides siamensis and a Parabdella sp. These leeches have a specialized structure called an "esophageal organ," the cells of which harbor bacterial symbionts. From the esophageal organ of each species, a 1.5-kb eubacterial 16S rRNA gene segment was amplified by PCR, cloned, and sequenced. Diagnostic PCR detected the symbiont in the esophageal organ and intestine. Phylogenetic analysis of the 16S rRNA gene(s) demonstrated that the symbionts from the leeches formed a monophyletic group in a well-defined clade containing endosymbiotic bacteria of plant sap-feeding insects in the gamma-subdivision of the Proteobacteria. The nucleotide compositions of the 16S rRNA gene from the leech symbionts were highly AT biased (53.7%).
摘要
我们对吸血舌蛭科的暹罗盘蛭（Placobdelloides siamensis）和一种拟蛭属（Parabdella sp.）的细胞内共生细菌进行了表征。这些水蛭具有一种名为“食管器官”的特殊结构，其细胞中含有细菌共生体。从每个物种的食管器官中，通过聚合酶链反应（PCR）扩增出一段1.5千碱基对（kb）的真细菌16S核糖体RNA（rRNA）基因片段，进行克隆和测序。诊断性PCR检测到食管器官和肠道中的共生体。对16S rRNA基因的系统发育分析表明，来自水蛭的共生体在一个明确的进化枝中形成了一个单系群，该进化枝包含变形菌门γ-亚纲中以植物汁液为食的昆虫的内共生细菌。来自水蛭共生体的16S rRNA基因的核苷酸组成具有高度的AT偏向性（53.7%）。
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