Araújo Irene Trigueiros, Fialho Alexandre M, de Assis Rosane M Santos, Rocha Mirna, Galvão Márcia, Cruz Cristiane M, Ferreira Monica S R, Leite José Paulo G
Laboratory of Comparative Virology, Department of Virology, Oswaldo Cruz Institute, Brazil.
J Trop Pediatr. 2002 Aug;48(4):214-8. doi: 10.1093/tropej/48.4.214.
Rotavirus strains from 91 patients treated at a children's hospital from 1996 to 1998 in Rio de Janeiro, Brazil, were characterized by electropherotyping, reverse transcription-PCR amplification for P and G genotypes, and Southern hybridization. Results obtained showed that following predominant [P],G type combination: P[4], G2 (21 per cent), P[8], G1 (17 per cent), P[8], G3 (13 per cent), which are prevalent throughout the world. However, an unexpected number of cases were associated with uncommon genotypes: P[8], G2 (13 per cent), P[8], G5 (11 per cent), P[8], G9 (7 per cent), P[8], G10 (4 per cent), P[6], G4 (3 per cent), P[6], G3 (1 per cent), P[4], G9 (1 per cent), and P[6], G9 (1 per cent). Mixed infections with more than one type were identified in only two cases and 16 per cent of the samples were not G and/or P typeable. A subset of G types was confirmed by Southern hybridization and chemiluminescent detection. Rotavirus seasonal distribution was observed between April and July. The contribution of the results obtained in the present investigation corroborates the required epidemiological surveillance for rotavirus infection in Brazil.
1996年至1998年期间，对巴西里约热内卢一家儿童医院收治的91例患者的轮状病毒毒株进行了电泳分型、P和G基因型的逆转录-聚合酶链反应扩增以及Southern杂交鉴定。结果显示，以下是主要的[P]、G型组合：P[4]，G2（21%）、P[8]，G1（17%）、P[8]，G3（13%），这些组合在全球都很常见。然而，意外的是，有一定数量的病例与不常见的基因型相关：P[8]，G2（13%）、P[8]，G5（11%）、P[8]，G9（7%）、P[8]，G10（4%）、P[6]，G4（3%）、P[6]，G3（1%）、P[4]，G9（1%）以及P[6]，G9（1%）。仅在两例中发现了一种以上类型的混合感染，16%的样本无法进行G和/或P分型。通过Southern杂交和化学发光检测对一部分G型进行了确认。轮状病毒的季节性分布在4月至7月之间观察到。本研究所得结果有助于巴西对轮状病毒感染进行必要的流行病学监测。
