Volotão Eduardo M, Soares Caroline C, Maranhão Adriana G, Rocha Ludmila N, Hoshino Yasutaka, Santos Norma
Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
J Med Virol. 2006 Feb;78(2):263-72. doi: 10.1002/jmv.20535.
Rotavirus diarrhea is a potentially life-threatening disease that affects millions of children annually around the world. Because protection against rotavirus disease is thought to be type specific, continuous rotavirus surveillance before and after implementation of a vaccine is still of essential importance. Rotavirus surveillance has been conducted in the city of Rio de Janeiro, Brazil since 1982. In the present study, we report rotavirus surveillance data in Rio de Janeiro city from 2000 to 2004. One hundred twenty nine of 1,568 (8.2%) stool samples, collected from children with acute diarrhea between January 2000 and July 2004 were rotavirus-positive. One hundred twenty eight of the 129 (99.2%) rotavirus-positive samples were genotyped for G and/or P specificity. G1 was the predominant strain (49.6%, 64/129) followed by G9 (30.2%, 39/129), and G4 (17.8%, 23/129); G2 and G3 viruses were not detected. One sample (0.8%) was non-typeable. P genotypes were determined for 124 of the 129 (96%) samples, and P[8] was the predominant genotype (90.6%, 117/129). Genotypes P[4] and P[9] were detected in two (1.6%) samples each; one (0.8%) sample presented P[6] genotype; and five (3.8%) samples were non-typeable. Two samples (1.6%) presented mixed P genotypes (P[6] + P[8]). Two unusual strains were isolated: a G8P[4] strain isolated from a non-hospitalized child with diarrhea and a G10P[9] strain isolated from a hospitalized child with diarrhea.
轮状病毒腹泻是一种可能危及生命的疾病，每年在全球影响数百万儿童。由于人们认为针对轮状病毒疾病的保护具有型特异性，因此在疫苗实施前后持续进行轮状病毒监测仍然至关重要。自1982年以来，巴西里约热内卢市一直在开展轮状病毒监测。在本研究中，我们报告了2000年至2004年里约热内卢市的轮状病毒监测数据。2000年1月至2004年7月期间，从患有急性腹泻的儿童中采集的1568份粪便样本中有129份（8.2%）轮状病毒呈阳性。129份轮状病毒阳性样本中的128份（99.2%）进行了G和/或P特异性基因分型。G1是主要毒株（49.6%，64/129），其次是G9（30.2%，39/129）和G4（17.8%，23/129）；未检测到G2和G3病毒。1份样本（0.8%）无法分型。129份样本中的124份（96%）确定了P基因型，P[8]是主要基因型（90.6%，117/129）。在两份样本（1.6%）中分别检测到P[4]和P[9]基因型；1份样本（0.8%）呈现P[6]基因型；5份样本（3.8%）无法分型。两份样本（1.6%）呈现混合P基因型（P[6]+P[8]）。分离出两株不寻常的毒株：一株从一名腹泻的非住院儿童中分离出的G8P[4]毒株和一株从一名腹泻的住院儿童中分离出的G10P[9]毒株。
