Miller M E, Howard D
Blood Cells. 1979 Aug;5(3):389-403.
The present studies were done to determine whether preventing the respiratory alkalosis, which is known to occur with acute "hypoxic" stimuli, would lead to alterations in plasma concentrations of erythropoietin (Ep). Rats were subjected to two acute stresses, hypoxia and blood loss, separately and in combination, with and without the added stress of hypercarbia. Hypercarbia in all experimental groups was associated with a decrease in plasma concentrations of Ep. This reduction in plasma Ep with hypercarbia could not be fully explained by the higher arterial pO2S or p50S of the hypercarbic rats. Hypercarbia may have indirectly suppressed Ep production by increasing blood flow to the site of Ep production. Alternatively, the cell of origin of Ep could be sensitive to changes in pH and/or PCO2. It was further demonstrated that neither the onset nor the degree of reticulocytosis could be predicted by the plasma Ep concentrations. It is likely that the removal of red blood cells led to a decrease in marrow transit time with the early emergence of reticulocytes after acute blood loss.
本研究旨在确定预防已知会伴随急性“低氧”刺激而发生的呼吸性碱中毒是否会导致促红细胞生成素（Ep）血浆浓度的改变。将大鼠分别单独或联合施加两种急性应激，即低氧和失血，并施加或不施加高碳酸血症这一额外应激。所有实验组中的高碳酸血症均与Ep血浆浓度降低相关。高碳酸血症大鼠较高的动脉血氧分压（pO2）或氧解离曲线中P50的升高并不能完全解释高碳酸血症时血浆Ep的这种降低。高碳酸血症可能通过增加流向Ep产生部位的血流量而间接抑制Ep的产生。或者，Ep的起源细胞可能对pH和/或二氧化碳分压（PCO2）的变化敏感。进一步证明，网织红细胞增多症的发生时间和程度均无法通过血浆Ep浓度来预测。急性失血后，红细胞的清除可能导致骨髓转运时间缩短，从而使网织红细胞提前出现。
