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Prevalence of Enterocytozoon bieneusi in swine: an 18-month survey at a slaughterhouse in Massachusetts.猪肠道内微小隐孢子虫的流行情况：在马萨诸塞州一家屠宰场进行的为期18个月的调查
Appl Environ Microbiol. 2002 May;68(5):2595-9. doi: 10.1128/AEM.68.5.2595-2599.2002.
2
Expression of the highly polymorphic Cryptosporidium parvum Cpgp40/15 gene in genotype I and II isolates.微小隐孢子虫高度多态性Cpgp40/15基因在I型和II型分离株中的表达
Mol Biochem Parasitol. 2002 Feb;119(2):203-15. doi: 10.1016/s0166-6851(01)00416-9.
3
Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigations.微小隐孢子虫基因特异性多态性快速鉴定方法的改进及其在临床和流行病学调查中的应用。
Appl Environ Microbiol. 2001 Dec;67(12):5581-4. doi: 10.1128/AEM.67.12.5581-5584.2001.
4
Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.用于扩增隐孢子虫卵囊壁蛋白基因的巢式聚合酶链反应
Emerg Infect Dis. 2001 Jan-Feb;7(1):49-56. doi: 10.3201/eid0701.700049.
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Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru.秘鲁利马儿童中5种隐孢子虫寄生虫的鉴定。
J Infect Dis. 2001 Feb 1;183(3):492-7. doi: 10.1086/318090. Epub 2000 Dec 20.
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Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites.隐孢子虫寄生虫卵囊壁蛋白基因诊断靶区域的序列差异。
Appl Environ Microbiol. 2000 Dec;66(12):5499-502. doi: 10.1128/AEM.66.12.5499-5502.2000.
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Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal samples from livestock animals.英国隐孢子虫属的分子流行病学分析：对1705份人类粪便样本和105份家畜粪便样本中的隐孢子虫属进行基因分型的结果
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9
Extensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis.通过多位点微卫星分析鉴定出微小隐孢子虫的广泛多态性。
Appl Environ Microbiol. 2000 Aug;66(8):3344-9. doi: 10.1128/AEM.66.8.3344-3349.2000.
10
Animal propagation and genomic survey of a genotype 1 isolate of Cryptosporidium parvum.微小隐孢子虫1型分离株的动物传播与基因组调查
Mol Biochem Parasitol. 2000 May;108(2):187-97. doi: 10.1016/s0166-6851(00)00211-5.

经不同宿主物种传代的微小隐孢子虫人基因型1分离株的遗传分析。

Genetic analysis of a Cryptosporidium parvum human genotype 1 isolate passaged through different host species.

作者信息

Akiyoshi D E, Feng X, Buckholt M A, Widmer G, Tzipori S

机构信息

Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.

出版信息

Infect Immun. 2002 Oct;70(10):5670-5. doi: 10.1128/IAI.70.10.5670-5675.2002.

DOI:10.1128/IAI.70.10.5670-5675.2002
PMID:12228296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC128364/
Abstract

Cryptosporidium parvum TU502, a genotype 1 isolate of human origin, was passaged through three different mammalian hosts, including humans, pigs, and calves. It was confirmed to be genotype 1 by PCR-restriction fragment length polymorphism analysis of the Cryptosporidium oocyst wall protein gene, direct sequencing of PCR fragments of the small subunit rRNA and beta-tubulin genes, and microsatellite analysis. This isolate was shown to be genetically stable when passaged through the three mammalian species, with no evidence of the emergence of new subpopulations as observed by a genotype-specific PCR assay. TU502 oocysts from different sources failed to infect gamma interferon knockout mice, a characteristic of genotype 1 isolates. The genotypic and phenotypic characterization of TU502 is significant since it is the isolate selected to sequence the genome of C. parvum genotype 1 and is currently used in several research projects including human volunteer studies.

摘要

微小隐孢子虫TU502是一种源自人类的1型基因型分离株，它在包括人类、猪和小牛在内的三种不同哺乳动物宿主中传代。通过对隐孢子虫卵囊壁蛋白基因进行PCR-限制性片段长度多态性分析、对小亚基rRNA和β-微管蛋白基因的PCR片段进行直接测序以及微卫星分析，证实其为1型基因型。当该分离株在这三种哺乳动物物种中传代时，显示出遗传稳定性，通过基因型特异性PCR检测未观察到新亚群出现的证据。来自不同来源的TU502卵囊未能感染γ干扰素基因敲除小鼠，这是1型基因型分离株的一个特征。TU502的基因型和表型特征具有重要意义，因为它是被选中对微小隐孢子虫1型基因型基因组进行测序的分离株，目前用于包括人体志愿者研究在内的多个研究项目。