Buckholt Michael A, Lee John H, Tzipori Saul
Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.
Appl Environ Microbiol. 2002 May;68(5):2595-9. doi: 10.1128/AEM.68.5.2595-2599.2002.
Slaughterhouse pig samples were analyzed by PCR for Enterocytozoon bieneusi infection. Thirty-two percent were found to be positive, with rates being higher over the summer months. Three isolates from pigs were identical in their ribosomal internal transcribed spacer sequence to human E. bieneusi type D, two were identical to type F (from a pig), and nine were previously unreported. The viability of these spores was demonstrated by their ability to infect gnotobiotic piglets. The presence of the infection in liver was shown by in situ hybridization.
采用聚合酶链反应(PCR)对屠宰场猪样本进行了微小隐孢子虫感染分析。结果发现32%的样本呈阳性,且夏季月份的感染率更高。从猪身上分离出的3个菌株,其核糖体内部转录间隔区序列与人类微小隐孢子虫D型相同,2个与F型(来自一头猪)相同,还有9个是以前未报告过的。这些孢子的活力通过其感染无菌仔猪的能力得到了证实。原位杂交显示肝脏中存在这种感染。