Spano F, Putignani L, McLauchlin J, Casemore D P, Crisanti A
Istituto di Parasskitologia, Università di Roma La Sapienza, Italy.
FEMS Microbiol Lett. 1997 May 15;150(2):209-17. doi: 10.1016/s0378-1097(97)00115-8.
Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species. We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptosporidium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. Within Wc-I, polymorphic Rsal restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections.
在隐孢子虫病自然爆发期间，从豚鼠体内分离出了怀氏隐孢子虫。尽管怀氏隐孢子虫在形态和抗原方面与微小隐孢子虫相似，但它表现出不同的宿主范围和感染部位，可能代表一个独立的物种或亚种。我们使用聚合酶链反应克隆了怀氏隐孢子虫编码隐孢子虫卵囊壁蛋白（COWP）的基因的两个不同的550 bp长的DNA片段，即Wc-I和Wc-II，它们与微小隐孢子虫的同源物有98%的同一性。在Wc-I内，利用多态性Rsal限制性酶切位点开发了一种聚合酶链反应-限制性片段长度多态性方法，该方法能够区分怀氏隐孢子虫和微小隐孢子虫，并识别出两组与动物和人类感染有不同关联的微小隐孢子虫分离株。
