羧甲基壳聚糖对正常皮肤成纤维细胞和瘢痕疙瘩皮肤成纤维细胞增殖及胶原分泌的影响

The effect of carboxymethyl-chitosan on proliferation and collagen secretion of normal and keloid skin fibroblasts.

作者信息

Chen Xi-Guang, Wang Zhen, Liu Wan-Shun, Park Hyun-Jin

机构信息

Graduate School of Biotechnology, Korea University, Sungbuk-Ku, Seoul, South Korea.

出版信息

Biomaterials. 2002 Dec;23(23):4609-14. doi: 10.1016/s0142-9612(02)00207-7.

DOI:10.1016/s0142-9612(02)00207-7

PMID:12322982

Abstract

In this study, different molecular weight CM-chitosans were prepared and the effects on the growth and collagen secretion of normal skin fibroblasts and keloid fibroblasts were investigated in vitro. CM-chitosan promoted the proliferation of the normal skin fibroblast significantly but inhibited the proliferation of keloid fibroblast. The higher CM-chitosan concentration had a higher initial effect and the lower CM-chitosan concentration had a longer affecting time to the normal skin fibroblast. The lower molecular weight CM-chitosan had significant twofold activities. The CM-chitosan could reduce the ratio of type I/III collagen in keloid fibroblast by inhibiting the secretion of collagen type I; and had no effect on the secretion of types I and III collagen in the normal skin fibroblast.

摘要

在本研究中，制备了不同分子量的羧甲基壳聚糖（CM-壳聚糖），并在体外研究了其对正常皮肤成纤维细胞和瘢痕疙瘩成纤维细胞生长及胶原蛋白分泌的影响。CM-壳聚糖显著促进了正常皮肤成纤维细胞的增殖，但抑制了瘢痕疙瘩成纤维细胞的增殖。较高浓度的CM-壳聚糖对正常皮肤成纤维细胞的初始作用更强，而较低浓度的CM-壳聚糖对其影响时间更长。较低分子量的CM-壳聚糖具有显著的双重活性。CM-壳聚糖可通过抑制I型胶原蛋白的分泌来降低瘢痕疙瘩成纤维细胞中I/III型胶原蛋白的比例；而对正常皮肤成纤维细胞中I型和III型胶原蛋白的分泌没有影响。