Holberg-Petersen Mona, Jenum Pål A, Mannsåker Turid, Melby Kjetil K
Department of Microbiology, Oslo University Hospital, Ullevål, Norway.
Scand J Infect Dis. 2011 Mar;43(3):221-4. doi: 10.3109/00365548.2010.538855. Epub 2010 Nov 25.
An in-house nested polymerase chain reaction (PCR) was prospectively compared with culture for Bordetella pertussis detection in 435 nasopharyngeal and/or throat swabs from 304 patients. One hundred specimens - 21% of nasopharyngeal swabs and 25% of throat swabs - were PCR- and/or culture-positive. Seventy percent of positive nasopharyngeal samples and 44% of positive throat samples were culture-positive.
对来自304例患者的435份鼻咽和/或咽喉拭子，将一种内部巢式聚合酶链反应（PCR）与用于检测百日咳博德特氏菌的培养法进行前瞻性比较。100份标本——21%的鼻咽拭子和25%的咽喉拭子——PCR和/或培养呈阳性。70%的鼻咽阳性样本和44%的咽喉阳性样本培养呈阳性。
