Dänicke S, Ueberschär K H, Halle I, Valenta H, Flachowsky G
Institute of Animal Nutrition, Federal Agricultural Research Centre (FAL), Braunschweig, Germany.
Arch Tierernahr. 2001;55(4):299-313. doi: 10.1080/17450390109386199.
Two experiments were carried out with male broilers to examine excretion kinetics of zearalenone (ZON) and its metabolites and their occurrence in blood plasma and bile fluid after a single oral dose of ZON (approximately 6 micrograms/kg BW) from naturally contaminated wheat (406 micrograms ZON per kg). In addition, this ZON bolus was administered either in the absence or presence of a detoxifying agent (Mycofix-Plus, Biomin GmbH, Herzogenburg, Austria). Specimens were sampled after administration of the zearalenone bolus at different times of up to 48 h. Excretion of zearalenone and alpha-zearalenol as the only detectable metabolite of ZON peaked at approximately 6.5 h after administration of the bolus. Cumulative excretion of both substances amounted to approximately 58% of ZON intake after 48 h, when a plateau was achieved. The incomplete recovery could have been due to a partial total degradation of ZON in the digestive tract, undetected sulfate conjugates of ZON or its metabolites, to other unknown and undetected metabolites or to incomplete analytical recovery from the matrix, and needs to be examined further. Peak concentrations of zearalenone and alpha-zearalenol in bile were detected in the time period of approximately 2 to 6 h after bolus, whereas ZON and metabolite concentrations in blood plasma were around or lower than the detection limits. Mycofix-Plus supplementation seemed to have only minor or no effects on the parameters examined.
进行了两项实验，以研究雄性肉鸡在单次口服来自天然污染小麦（每千克含406微克玉米赤霉烯酮）的玉米赤霉烯酮（ZON，约6微克/千克体重）后，玉米赤霉烯酮及其代谢产物的排泄动力学，以及它们在血浆和胆汁中的出现情况。此外，在不存在或存在解毒剂（Mycofix-Plus，奥地利黑措根堡Biomin有限公司）的情况下给予这种玉米赤霉烯酮大剂量药物。在给予玉米赤霉烯酮大剂量药物后的不同时间（最长48小时）采集样本。玉米赤霉烯酮和α-玉米赤霉醇（作为玉米赤霉烯酮唯一可检测到的代谢产物）的排泄在给予大剂量药物后约6.5小时达到峰值。48小时后达到平台期时，两种物质的累积排泄量约占玉米赤霉烯酮摄入量的58%。回收率不完全可能是由于玉米赤霉烯酮在消化道中部分完全降解、未检测到的玉米赤霉烯酮或其代谢产物的硫酸酯结合物、其他未知且未检测到的代谢产物，或从基质中的分析回收率不完全，需要进一步研究。在给予大剂量药物后约2至6小时的时间段内检测到胆汁中玉米赤霉烯酮和α-玉米赤霉醇的峰值浓度，而血浆中玉米赤霉烯酮和代谢产物的浓度约为检测限或低于检测限。补充Mycofix-Plus似乎对所检测的参数只有轻微影响或没有影响。
