Yumita Nagahiko, Kawabata Ken-ichi, Sasaki Kazuaki, Umemura Shin-ichiro
School of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Ultrason Sonochem. 2002 Oct;9(5):259-65. doi: 10.1016/s1350-4177(02)00080-9.
The ultrasonically induced cytotoxic effect of erythrosin B (EB) on isolated sarcoma 180 cells was investigated. The tumor cells were suspended in an air-saturated phosphate buffered saline and exposed to ultrasound at 1.93 MHz in a standing-wave mode for up to 60 s in the presence and absence of EB. The rate of cell damage induction by ultrasound was enhanced by 4-5 times with 160-microM EB, while no cell damage was observed with EB alone. This enhancement was significantly inhibited by histidine. Sonochemical generation of active oxygen species in the presence of EB, measured by ESR spectroscopy, was also inhibited by histidine. These results indicate the involvement of a sonochemical mechanism.
研究了赤藓红B（EB）超声诱导的对分离的肉瘤180细胞的细胞毒性作用。将肿瘤细胞悬浮于空气饱和的磷酸盐缓冲盐水中，在有和没有EB存在的情况下，以驻波模式于1.93MHz下暴露于超声长达60秒。160微摩尔的EB使超声诱导细胞损伤的速率提高了4至5倍，而单独使用EB时未观察到细胞损伤。组氨酸可显著抑制这种增强作用。通过电子自旋共振光谱法测量，在EB存在下活性氧物种的声化学产生也受到组氨酸的抑制。这些结果表明涉及声化学机制。
