Zhou Yuyong, Wu Rui, Zhao Qin, Chang Yung-Fu, Wen Xintian, Feng Yao, Huang Xiaobo, Wen Yiping, Yan Qigui, Huang Yong, Ma Xiaoping, Han Xinfeng, Cao Sanjie
Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Sichuan Science-Observation Experiment of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, 611130, China.
Arch Virol. 2018 May;163(5):1351-1355. doi: 10.1007/s00705-018-3765-2. Epub 2018 Feb 6.
Previously, we isolated the Japanese encephalitis virus (JEV) strain SCYA201201. In this study, we passed the SCYA201201 strain in Syrian baby hamster kidney (BHK-21) cells 120 times to obtain the SCYA201201-0901 strain, which exhibited an attenuated phenotype in mice. Comparison of SCYA201201-0901 amino acid sequences with those of other JEV strains revealed a single mutation, I176R, in the E coding region. Using reverse genetic technology, we provide evidence that this single E-I176R mutation does not affect virus growth in BHK-21 cells but significantly decreases JEV neurovirulence in mice. This study provides critical information for understanding the molecular mechanism of JEV attenuation.
此前，我们分离出了日本脑炎病毒（JEV）株SCYA201201。在本研究中，我们将SCYA201201株在叙利亚幼仓鼠肾（BHK-21）细胞中传代120次，获得了SCYA201201-0901株，该毒株在小鼠中表现出减毒表型。将SCYA201201-0901的氨基酸序列与其他JEV株的序列进行比较，发现在E编码区有一个单一突变I176R。利用反向遗传技术，我们提供了证据表明，这一单一的E-I176R突变不影响病毒在BHK-21细胞中的生长，但显著降低了JEV在小鼠中的神经毒力。本研究为理解JEV减毒的分子机制提供了关键信息。
