Makino S, Jenkin H M, Yu H M, Townsend D
J Bacteriol. 1970 Jul;103(1):62-70. doi: 10.1128/jb.103.1.62-70.1970.
The lipid compositions of (i) monkey kidney (MK-2) cells cultivated in Eagle's minimal essential medium (MEM) with 5% calf serum, (ii) MK-2 cells cultivated in Waymouth medium supplemented with 20 mug of sodium oleate and 2 mg of bovine albumin per ml, (iii) Chlamydia psittaci strain 6BC grown in the latter host system, and (iv) calf serum were compared. Strain 6BC contains 31% phosphatidyl ethanolamine (PE) and 15% phosphatidyl glycerol (PG), whereas the host cell contains almost the same amount of PE (27%) and no PG. A high concentration of total lipid was observed in strain 6BC (29 to 34%), whereas MK-2 cells contain only 9 to 15% and calf serum contains 4.5% total lipid. The fatty acids of the total lipid from strain 6BC contain branched-chain acids. These fatty acids were found mostly in PE (33.0%) and PG (37.0%). No branched-chain fatty acid was found in the MK-2 cells. There was an increase in triglyceride content when MK-2 cells cultivated in MEM (19.2%) were compared with cells cultivated in Waymouth medium (28.0%). A high concentration (62.0%) of octadecenoic acid (C18:1) was found in the triglyceride of MK-2 cells cultivated in Waymouth medium. The level of polyunsaturated fatty acids observed in MK-2 cells cultivated in Waymouth medium (10.8%) and in the chlamydiae grown in these cells (13.3%) was low compared with the level in MK-2 cells (28.8%) cultivated in MEM with 5% calf serum and the level in calf serum itself (50.8%). A higher ratio of sterol ester to free sterol was found in calf serum than in MK-2 cells or in chlamydiae. Host contribution to lipid composition of strain 6BC is discussed.
(i) 在含有5%小牛血清的伊格尔氏基本培养基（MEM）中培养的猴肾（MK - 2）细胞；(ii) 在添加了每毫升20微克油酸钠和2毫克牛白蛋白的韦茅斯培养基中培养的MK - 2细胞；(iii) 在后者宿主系统中生长的鹦鹉热衣原体6BC菌株；(iv) 小牛血清。6BC菌株含有31%的磷脂酰乙醇胺（PE）和15%的磷脂酰甘油（PG），而宿主细胞含有几乎相同量的PE（27%）且不含PG。在6BC菌株中观察到总脂质浓度较高（29%至34%），而MK - 2细胞仅含有9%至15%，小牛血清含有4.5%的总脂质。6BC菌株总脂质中的脂肪酸含有支链酸。这些脂肪酸大多存在于PE（33.0%）和PG（37.0%）中。在MK - 2细胞中未发现支链脂肪酸。当将在MEM中培养的MK - 2细胞（19.2%）与在韦茅斯培养基中培养的细胞（28.0%）进行比较时，甘油三酯含量有所增加。在韦茅斯培养基中培养的MK - 2细胞的甘油三酯中发现了高浓度（62.0%）的十八碳烯酸（C18:1）。与在含有5%小牛血清的MEM中培养的MK - 2细胞（28.8%）以及小牛血清本身（50.8%）中的水平相比，在韦茅斯培养基中培养的MK - 2细胞（10.8%）和在这些细胞中生长的衣原体（13.3%）中观察到的多不饱和脂肪酸水平较低。在小牛血清中发现的甾醇酯与游离甾醇的比例高于MK - 2细胞或衣原体。讨论了宿主对6BC菌株脂质组成的贡献。
