Moran J, van Rijswijk B, Traicevski V, Kitajima E W, Mackenzie A M, Gibbs A J
Agriculture Victoria, Institute for Horticultural Development, Knoxfield, Vic., Australia.
Arch Virol. 2002 Oct;147(10):1855-67. doi: 10.1007/s00705-002-0865-8.
Three potyviruses were identified by gene sequencing and found to be widespread in species of Apiaceae in Australia. Only celery mosaic virus was found in celery crops and in one of 180 specimens of feral carrot ( Daucus carota). Another related but distinct novel potyvirus, carrot virus Y, was the only virus found in carrot crops and all except one feral carrot. A more distantly related novel potyvirus, apium virus Y, was found in plants of sea celery ( Apium prostratum), cultivated parsley ( Petroselinum crispum) and the immigrant weed species poison hemlock ( Conium maculatum). These three potyviruses, together with celery yellow mosaic virus of South America and the closely related carrot thin leaf virus and carrot virus B of North America, form a distinct subgenus of the Potyviridae most closely related to turnip mosaic virus and two potyviruses of yam; yam mosaic virus from the Ivory Coast and Japanese yam mosaic virus. Celery mosaic and carrot virus Y are probably recent migrants to Australia, but apium virus Y may have been endemic longer. In ELISA tests using polyclonal antibodies against virions of celery mosaic virus, some isolates of carrot virus Y were indistinguishable from celery mosaic virus, whereas others gave smaller absorbancy values, and those of apium virus Y did not react. This study shows the value of virus identification based on gene sequencing for planning control measures.
通过基因测序鉴定出三种马铃薯Y病毒，发现它们在澳大利亚的伞形科植物中广泛存在。仅在芹菜作物和180份野生胡萝卜（野胡萝卜）样本中的一份中发现了芹菜花叶病毒。另一种相关但不同的新型马铃薯Y病毒——胡萝卜Y病毒，是在胡萝卜作物以及除一份之外的所有野生胡萝卜中发现的唯一病毒。一种亲缘关系更远的新型马铃薯Y病毒——海芹菜Y病毒，在海芹菜（匍匐芹）、栽培欧芹（皱叶欧芹）和外来杂草物种毒芹（毒参）的植株中被发现。这三种马铃薯Y病毒，与南美洲的芹菜黄花叶病毒以及北美洲密切相关的胡萝卜细叶病毒和胡萝卜病毒B一起，构成了马铃薯Y病毒科中一个独特的亚属，与芜菁花叶病毒以及两种山药病毒关系最为密切；来自象牙海岸的山药花叶病毒和日本山药花叶病毒。芹菜花叶病毒和胡萝卜Y病毒可能是最近才传入澳大利亚的，但海芹菜Y病毒可能已经在当地存在了更长时间。在使用针对芹菜花叶病毒病毒粒子的多克隆抗体进行的酶联免疫吸附测定（ELISA）试验中，一些胡萝卜Y病毒分离株与芹菜花叶病毒无法区分，而其他分离株的吸光度值较小，海芹菜Y病毒的分离株则没有反应。这项研究表明了基于基因测序进行病毒鉴定对于规划控制措施的价值。
