通过热泳耗竭和对流捕获DNA。
Trapping of DNA by thermophoretic depletion and convection.
作者信息
Braun Dieter, Libchaber Albert
机构信息
Center for Studies in Physics and Biology, Rockefeller University, New York, New York 10021, USA.
出版信息
Phys Rev Lett. 2002 Oct 28;89(18):188103. doi: 10.1103/PhysRevLett.89.188103. Epub 2002 Oct 14.
Thermophoresis depletes DNA from a heated spot. We quantify for the first time the thermal diffusion constant D(T)=0.4x10(-8) cm(2)/s K for DNA, using fluorescent dyes and laser heating. For 5 kB DNA we extrapolate a 1000-fold depletion from a temperature difference of 50 K. Surprisingly, convection generated by the same heating can turn the depletion into trapping of DNA. Trapped DNA can form point geometries 20 microm in diameter with more than 1000-fold enhanced concentrations. The accumulation is driven only by temperature gradients and offers a new approach to biological microfluidics and replicating systems in prebiotic evolution.
热泳使加热点处的DNA耗尽。我们首次使用荧光染料和激光加热对DNA的热扩散常数D(T)=0.4×10⁻⁸ cm²/s·K进行了量化。对于5千碱基对的DNA，我们推断在50 K的温差下会有1000倍的耗尽。令人惊讶的是，相同加热产生的对流会使DNA的耗尽转变为捕获。被捕获的DNA可以形成直径为20微米的点状结构，其浓度提高了1000倍以上。这种积累仅由温度梯度驱动，为生物微流体和前生物进化中的复制系统提供了一种新方法。
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