Hnatyszyn O, Miño J, Ferraro G, Acevedo C
Cátedra de Farmacognosia, IQUIMEFA (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Phytomedicine. 2002 Sep;9(6):556-9. doi: 10.1078/09447110260573209.
Phyllanthus sellowianus Müller Arg. (Euphorbiaceae) is a plant used in folk medicine as a hypoglycemic and diuretic agent. The present study describes the hypoglycemic effect of fractions obtained from the stem barks of P. sellowianus using a bioassay-guided fractionation protocol and streptozotocin-induced hyperglycemic mice. The aqueous extract was partitioned between dichloromethane and butanol to yield the dichloromethane (D), butanol (B) and the remaining aqueous (A) fractions. Fractions B and A, administered at the dose of 200 mg/kg p.o., caused a significant reduction in blood glucose concentration at 6 and 9 h, while the same dose of fraction D was ineffective. The reduction in blood glucose levels obtained with the B and A fractions was similar to that observed with glibenclamide (10 mg/kg) which was used as a reference for the hypoglycemic activity. Phytochemical analysis of fractions B and A revealed the presence of flavonoid compounds, of which rutin and isoquercitrin were the major constituents, respectively. The possible involvement of these flavonoids in the hypoglycemic effect of the active fractions is discussed.
塞洛叶下珠（Phyllanthus sellowianus Müller Arg.，大戟科）是一种在民间医学中用作降血糖和利尿剂的植物。本研究采用生物测定导向的分级分离方案，以链脲佐菌素诱导的高血糖小鼠为模型，描述了从塞洛叶下珠茎皮中获得的各馏分的降血糖作用。水提取物在二氯甲烷和丁醇之间进行分配，得到二氯甲烷（D）、丁醇（B）和剩余的水相（A）馏分。以200 mg/kg口服给药的B馏分和A馏分在6小时和9小时时可显著降低血糖浓度，而相同剂量的D馏分则无效。B馏分和A馏分导致的血糖水平降低与用作降血糖活性参考的格列本脲（10 mg/kg）所观察到的情况相似。对B馏分和A馏分的植物化学分析表明存在黄酮类化合物，其中芦丁和异槲皮苷分别为主要成分。讨论了这些黄酮类化合物可能参与活性馏分降血糖作用的情况。
