Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
Biomed Res Int. 2013;2013:591313. doi: 10.1155/2013/591313. Epub 2013 Nov 4.
Annona genus contains plants used in folk medicine for the treatment of diabetes. In the present study, an aqueous extract prepared from Annona macroprophyllata (Annonaceae, also known as A. diversifolia) leaves was evaluated on both the activity of yeast α -glucosidase (an in vitro assay) and sucrose tolerance in Wistar rats. The results have shown that the aqueous extract from A. macroprophyllata inhibits the yeast α -glucosidase with an IC₅₀ = 1.18 mg/mL, in a competitive manner with a K(i) = 0.97 mg/mL, a similar value to that of acarbose (K(i) = 0.79 mg/mL). The inhibitory activity of A. macroprophyllata was reinforced by its antihyperglycemic effect, at doses of 100, 300, and 500 mg/kg in rats. Chromatographic analysis identified the flavonoids rutin and isoquercitrin in the most polar fractions of A. macroprophyllata crude extract, suggesting that these flavonoids are part of the active constituents in the plant. Our results support the use of A. macroprophyllata in Mexican folk medicine to control postprandial glycemia in people with diabetes mellitus, involving active constituents of flavonoid nature.
番荔枝属植物被民间医学用于治疗糖尿病。在本研究中，评估了番荔枝（番荔枝科，也称为 A. diversifolia）叶的水提物对酵母α-葡萄糖苷酶（体外测定）活性和 Wistar 大鼠蔗糖耐量的影响。结果表明，番荔枝叶的水提物以竞争性方式抑制酵母α-葡萄糖苷酶，IC₅₀=1.18mg/mL，K(i)=0.97mg/mL，与阿卡波糖（K(i)=0.79mg/mL）相似。番荔枝的抑制活性通过其在 100、300 和 500mg/kg 剂量下的抗高血糖作用得到加强。在番荔枝粗提取物的最极性部分中，通过色谱分析鉴定出了黄酮类化合物芦丁和异槲皮苷，表明这些黄酮类化合物是该植物中活性成分的一部分。我们的结果支持在墨西哥民间医学中使用番荔枝来控制糖尿病患者的餐后血糖，涉及黄酮类性质的活性成分。
