Heidelberg J F, Heidelberg K B, Colwell R R
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA.
Appl Environ Microbiol. 2002 Nov;68(11):5498-507. doi: 10.1128/AEM.68.11.5498-5507.2002.
The seasonal abundance of gamma-subclass Proteobacteria, Vibrio-Photobacterium, Vibrio cholerae-Vibrio mimicus, Vibrio cincinnatiensis, and Vibrio vulnificus in the Choptank River of Chesapeake Bay associated with zooplankton was monitored from April to December 1996. Large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. Large populations of bacteria were found to be associated with both the large and small size classes of zooplankton. Also, the species of bacteria associated with the zooplankton showed seasonal abundance, with the largest numbers occurring in the early spring and again in the summer, when zooplankton total numbers were correspondingly large. Approximately 0.01 to 40.0% of the total water column bacteria were associated with zooplankton, with the percentage of the total water column bacteria population associated with zooplankton varying by season. A taxonomically diverse group of bacteria was associated with zooplankton, and a larger proportion was found in and on zooplankton during the cooler months of the year, with selected taxa comprising a larger percent of the Bacteria in the summer. V. cholerae-V. mimicus and V. vulnificus comprised the bulk of the large and small zooplankton-associated Vibrio-Photobacterium species. In contrast, V. cincinnatiensis accounted for less than 0.1 to 3%. It is concluded that water column and zooplankton bacterial populations vary independently with respect to species composition since no correlation was observed between taxa occurring with highest frequency in the water column and those in association with zooplankton.
1996年4月至12月，对切萨皮克湾乔普坦克河中与浮游动物相关的γ-变形菌纲、弧菌-发光杆菌属、霍乱弧菌-拟态弧菌、辛辛那提弧菌和创伤弧菌的季节性丰度进行了监测。采集了大型（>202微米）和小型（64至202微米）浮游动物，通过荧光寡核苷酸直接计数法对与每个浮游动物大小类群相关的细菌进行计数。发现大量细菌与大型和小型浮游动物都有关联。此外，与浮游动物相关的细菌种类呈现季节性丰度，数量最多的时候出现在早春和夏季，此时浮游动物总数也相应较多。浮游动物相关细菌占水柱细菌总数的约0.01%至40.0%，且与浮游动物相关的水柱细菌总数百分比随季节变化。与浮游动物相关的细菌种类繁多，在一年中较凉爽的月份，在浮游动物体内和体表发现的细菌比例更大，而在夏季，某些分类群在细菌中所占百分比更大。霍乱弧菌-拟态弧菌和创伤弧菌占与大型和小型浮游动物相关的弧菌-发光杆菌属物种的大部分。相比之下，辛辛那提弧菌占比不到0.1%至3%。得出的结论是，水柱和浮游动物细菌种群在物种组成方面独立变化，因为在水柱中出现频率最高的分类群与与浮游动物相关的分类群之间未观察到相关性。
